Record Details

Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model.
Not Available
 
Creator Ghoke SS, Sood R, Kumar N, Pateriya AK, Bhatia S, Mishra A, Dixit R, Singh VK, Desai DN, Kulkarni DD, Dimri U,Singh VP. ilable
 
Subject O. sanctum, A. arabica, Avian influenza H9N2, in ovo testing, HA, Real time RT-qPCR
 
Description Not Available
Background
In the view of endemic avian influenza H9N2 infection in poultry, its zoonotic potential and emergence of antiviral resistance, two herbal plants, Ocimum sanctum and Acacia arabica, which are easily available throughout various geographical locations in India were taken up to study their antiviral activity against H9N2 virus. We evaluated antiviral efficacy of three different extracts each from leaves of O. sanctum (crude extract, terpenoid and polyphenol) and A. arabica (crude extract, flavonoid and polyphenol) against H9N2 virus using in ovo model.

Methods
The antiviral efficacy of different leaves extracts was systematically studied in three experimental protocols viz. virucidal (dose-dependent), therapeutic (time-dependent) and prophylactic (dose-dependent) activity employing in ovo model. The maximum non-toxic concentration of each herbal extracts of O. sanctum and A. arabica in the specific pathogen free embryonated chicken eggs was estimated and their antiviral efficacy was determined in terms of reduction in viral titres, measured by Haemagglutination (HA) and real time quantitative reverse transcription polymerase chain reaction (RT-qPCR) assays.

Results
All the extracts of O. sanctum (crude extract, terpenoid and polyphenol) and A. arabica (crude extract, flavonoid and polyphenol) showed significant virucidal activity, however, crude extractocimum and terpenoidocimum showed highly significant to significant (p < 0.001–0.01) decrease in virus genome copy numbers with lowest dose tested. Similarly, therapeutic effect was observed in all three extracts of O. sanctum in comparison to the virus control, nevertheless, crude extractocimum and terpenoidocimum maintained this effect for longer period of time (up to 72 h post-incubation). None of the leaves extracts of A. arabica had therapeutic effect at 24 and 48 h post-incubation, however, only the crude extractacacia and polyphenolacacia showed delayed therapeutic effect (72 h post-inoculation). Prophylactic potential was observed in polyphenolacacia with highly significant antiviral activity compared to virus control (p 
Not Available
 
Date 2020-08-05T10:24:06Z
2020-08-05T10:24:06Z
2018-07-01
 
Type Article
 
Identifier Ghoke, S. S., Sood, R., Kumar, N., Pateriya, A. K., Bhatia, S., Mishra, A., Dixit, R., Singh, V. K., Desai, D. N., Kulkarni, D. D., Dimri, U., & Singh, V. P. (2018). Evaluation of antiviral activity of Ocimum sanctum and Acacia arabica leaves extracts against H9N2 virus using embryonated chicken egg model. BMC complementary and alternative medicine, 18(1), 174.
2662-7671
http://krishi.icar.gov.in/jspui/handle/123456789/38943
 
Language English
 
Relation Not Available;
 
Publisher Springer Nature