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MicroRNA hsa-miR-324-5p Suppresses H5N1 Virus Replication by Targeting the Viral PB1 and Host CUEDC2

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Title MicroRNA hsa-miR-324-5p Suppresses H5N1 Virus Replication by Targeting the Viral PB1 and Host CUEDC2
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Creator Kumar, A., Kumar, A., Ingle, H., Kumar, S., Mishra, R., Verma, M. K., Biswas, D., Kumar, N. S., Mishra, A., Raut, A. A., Takaoka, A., & Kumar, H.
 
Subject antiviral immunity; avian viruses; innate immunity; miRNA; viral RNA polymerase
 
Description Not Available
MicroRNAs (miRNAs) are small noncoding RNAs that are crucial posttranscriptional regulators for host mRNAs. Recent studies indicate that miRNAs may modulate host response during RNA virus infection. However, the role of miRNAs in immune response against H5N1 infection is not clearly understood. In this study, we showed that expression of cellular miRNA miR-324-5p was downregulated in A549 cells in response to infection with RNA viruses H5N1, A/PR8/H1N1, and Newcastle disease virus (NDV) and transfection with poly(I·C). We found that miR-324-5p inhibited H5N1 replication by targeting the PB1 viral RNA of H5N1 in host cells. In addition, transcriptome analysis revealed that miR-324-5p enhanced the expression of type I interferon, type III interferon, and interferon-inducible genes (ISGs) by targeting CUEDC2, the negative regulator of the JAK1-STAT3 pathway. Together, these findings highlight that the miR-324-5p plays a crucial role in host defense against H5N1 by targeting viral PB1 and host CUEDC2 to inhibit H5N1 replication.IMPORTANCE Highly pathogenic influenza A virus (HPAIV) continues to pose a pandemic threat globally. From 2003 to 2017, H5N1 HPAIV caused 453 human deaths, giving it a high mortality rate (52.74%). This work shows that miR-324-5p suppresses H5N1 HPAIV replication by directly targeting the viral genome (thereby inhibiting viral gene expression) and cellular CUEDC2 gene, the negative regulator of the interferon pathway (thereby enhancing antiviral genes). Our study enhances the knowledge of the role of microRNAs in the cellular response to viral infection. Also, the study provides help in understanding how the host cells utilize small RNAs in controlling the viral burden.
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Date 2020-08-05T10:34:13Z
2020-08-05T10:34:13Z
2018-10-01
 
Type Article
 
Identifier Kumar, A., Kumar, A., Ingle, H., Kumar, S., Mishra, R., Verma, M. K., Biswas, D., Kumar, N. S., Mishra, A., Raut, A. A., Takaoka, A., & Kumar, H. (2018). MicroRNA hsa-miR-324-5p Suppresses H5N1 Virus Replication by Targeting the Viral PB1 and Host CUEDC2. Journal of virology, 92(19), e01057-18.
0022-538X
http://krishi.icar.gov.in/jspui/handle/123456789/38945
 
Language English
 
Relation Not Available;
 
Publisher American Society for Microbiology