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Antioxidant capcity, lipid oxidation status and expression of specific selenoprotein mRNA in Longissimus dorsi muscle in lambs (Ovies aries) supplemented with supranutritional selenium

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Title Antioxidant capcity, lipid oxidation status and expression of specific selenoprotein mRNA in Longissimus dorsi muscle in lambs (Ovies aries) supplemented with supranutritional selenium
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Creator Nongkhlaw SS
Suganthi RU
Ghosh J
Malik PK
Awachat VB
Krishnamoorthy P
Pal DT
 
Subject Antioxidant
Lambs
Lipid oxidation
Longissimus dorsi muscle
Selenoprotein genes
Supranutritional selenium
 
Description Not Available
Selenium is known to play a key role in maintenance of redox status of tissues, immunity, reproduction, thyroid
and muscle functions. The present investigation was carried out to understand the effect of supranutritional dietary Se supplementation on antioxidant capacity, lipid oxidation and expression of specific selenoprotein mRNA in Longissimus dorsi muscles of growing lambs. Twenty male lambs of 5–6 months of age were fed basal diet supplemented with 0.5, 1.5 or 4.5 ppm Se-yeast (organic Se) or without Se (control) for 90 days. The antioxidant capacity, lipid oxidation of meat during different days of storage and the mRNA expression of GPX1, GPX2, GPX3, TXNRD1, TXNRD3, DIO1, DIO2, DIO3, SEPP1, SEP15 and SEPW1 were studied in Longissimus dorsi muscles of sheep. The results indicated improvement in antioxidant status by supplementation of 1.5 and 4.5 ppm Se, and reduction in meat lipid oxidation status on day 0 without any further reduction after 3 and 7 days of storage in all the Se supplemented lambs. A selective change in expression of GPX2, GPX3, TXNRD1, DIO2, DIO3, SEPP1, SEP15 and SEPW1 mRNA was observed by supranutritional Se while GPX1, TXNRD3, DIO1 and expressions remained unaffected by supplementation. In conclusion, supranutritional Se supplementation in lambs increased antioxidant status, reduced lipid oxidation status with limited effect on oxidative stability of meat during storage and regulated Longissimus dorsi muscle selenoprotein mRNA expression differentially depending on the Se feeding levels. Our results thus provided new insights into the regulation of selenoprotein gene expression by supranutritional levels of dietary Se.
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Date 2019-09-30T11:34:37Z
2019-09-30T11:34:37Z
2019-09-26
 
Type Research Paper
 
Identifier Not Available
0367-8318
http://krishi.icar.gov.in/jspui/handle/123456789/23109
 
Language English
 
Relation Not Available;
 
Publisher Indian Council of Agricultural Research