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Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis

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Title Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
 
Creator Binsila KB
Selvaraju S
Ghosh SK
Parthipan S
Archana SS
Arangasamy A
Prasad JK
Bhatta R,
Ravindra JP
 
Subject ECM enrichment
Enzymatic isolation
Ovine testis
Spermatogonial stem cell
 
Description Not Available
The present study aimed to isolate and enrich putative SSCs from ram testes, which are positive for promyelocytic leukaemia zinc-finger protein (PLZF). The putative SSCs were isolated using a combination of enzymes with different concentrations, collagenase (1 and 2 mg/ml), hyaluronidase (1 mg/ml) and trypsin (0.25 and 0.5 mg/ml). The isolated SSCs were purified using an extracellular matrix such as laminin (20 μg/ml), DSA-lectin (5 μg/ml) and gelatin (0.2%) in combination with BSA (0.5 mg/ml). The number of putative SSCs/ tubule was significantly (p 
ICAR-NIANP
 
Date 2019-11-15T06:48:33Z
2019-11-15T06:48:33Z
2018-04-18
 
Type Research Paper
 
Identifier Binsila BK, Selvaraju S, Ghosh SK, Parthipan S, Archana SS, Arangasamy A, Prasad JK, Bhatta R, Ravindra JP. 2018. Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis. Animal Reproduction Science, 196:9-18
0378-4320
http://krishi.icar.gov.in/jspui/handle/123456789/24754
 
Language English
 
Relation Not Available;
 
Publisher Elsevier