Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
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Title |
Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis
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Creator |
Binsila KB
Selvaraju S Ghosh SK Parthipan S Archana SS Arangasamy A Prasad JK Bhatta R, Ravindra JP |
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Subject |
ECM enrichment
Enzymatic isolation Ovine testis Spermatogonial stem cell |
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Description |
Not Available
The present study aimed to isolate and enrich putative SSCs from ram testes, which are positive for promyelocytic leukaemia zinc-finger protein (PLZF). The putative SSCs were isolated using a combination of enzymes with different concentrations, collagenase (1 and 2 mg/ml), hyaluronidase (1 mg/ml) and trypsin (0.25 and 0.5 mg/ml). The isolated SSCs were purified using an extracellular matrix such as laminin (20 μg/ml), DSA-lectin (5 μg/ml) and gelatin (0.2%) in combination with BSA (0.5 mg/ml). The number of putative SSCs/ tubule was significantly (p ICAR-NIANP |
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Date |
2019-11-15T06:48:33Z
2019-11-15T06:48:33Z 2018-04-18 |
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Type |
Research Paper
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Identifier |
Binsila BK, Selvaraju S, Ghosh SK, Parthipan S, Archana SS, Arangasamy A, Prasad JK, Bhatta R, Ravindra JP. 2018. Isolation and enrichment of putative spermatogonial stem cells from ram (Ovis aries) testis. Animal Reproduction Science, 196:9-18
0378-4320 http://krishi.icar.gov.in/jspui/handle/123456789/24754 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier
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