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Asthenozoospermic buffalo (Bubalus bubalis) sperm tail is associated with lower expression of tektin-2 and Spag6 and higher expression of specific tyrosine phosphorylated proteins.

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Title Asthenozoospermic buffalo (Bubalus bubalis) sperm tail is associated with lower expression of tektin-2 and Spag6 and higher expression of specific tyrosine phosphorylated proteins.
Not Available
 
Creator B. C. Divyashree
Sudhir C. Roy
Vijayasaraswathy S. Gurupriya
 
Subject Asthenozoospermia
Buffalo
Motility
SPAG6
Sperm
Tektin-2
Tyrosine phosphorylated proteins
 
Description Not Available
Study of motility-associated proteins is a direct approach to decode the underlying mechanisms of the
motility-regulation pathway in a mammalian spermatozoon. In this aspect, attempts were made to
characterize and study the expression pattern of two candidate motility-associated proteins of
mammalian sperm such as Tektin-2 and SPAG6 and post-translationally modified tyrosine
phosphorylated proteins between the two defined groups of buffaloes: normozoospermic (NS) and
asthenozoospermic (AS). 1-D Western blot demonstrated three Tektin-2 immunoreactive bands (95.3,
55.7 and 33.0 kDa) in buffalo sperm tails. Of these, the intensity of 95.3 kDa band reduced significantly
(P ≤ 0.05) in sperm tail of AS group as compared to that of NS group. Similarly, the sperm tail
demonstrated a single SPAG6-immnunoreactive band of 31.6 kDa that reduced significantly (P ≤ 0.05)
in sperm tail of AS group as compared to that of NS group. Both AS and NS group sperm tail
demonstrated at least five tyrosine phosphorylated proteins (89.5, 53.3, 44.5, 30.9 and 16.7 kDa). Of
these, the intensity of 44.5 kDa band was significantly higher (P ≤ 0.05) in AS group as compared to
that of NS group. Interestingly, within the AS group, degree of expression of 53.3 kDa tyrosine
phosphorylated protein was bull-specific. The findings of the present study have demonstrated that AS
group of buffalo bulls are associated with lower expression of Tektin-2 and SPAG6 and higher
expression of specific tyrosine phosphorylated proteins.
Department of Biotechnology (DBT), Govt. of India (Grant No. BT/PR3595/AAQ/1/482/2011)
 
Date 2019-11-15T06:50:56Z
2019-11-15T06:50:56Z
2019-02-15
 
Type Research Paper
 
Identifier Divyashree BC, Roy SC, Gurupirya VS. 2019. Asthenozoospermic buffalo (Bubalus bubalis) sperm tail is associated with lower expression of tektin-2 and Spag6 and higher expression of specific tyrosine phosphorylated proteins. Journal of Experimental Biology and Agricultural Sciences, 7:86-94.
2320 – 8694
http://krishi.icar.gov.in/jspui/handle/123456789/24756
 
Language English
 
Relation Not Available;
 
Publisher Horizon Publisher India