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Expression of ADAMTS10 in male reproductive tract of buffaloes (Bubalus bubalis)

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Title Expression of ADAMTS10 in male reproductive tract of buffaloes (Bubalus bubalis)
Not Available
 
Creator Gurupriya VS,
Roy SC,
Javvaji PK,
Dhali A,
Badami S,
Rahim F,
Divyashree BC,
Panda AP
 
Subject ADAMTS10
Buffalo
Gene expression
Male reproductive tract Sperm
 
Description Not Available
Large number of proteases have been identified in different parts of the male reproductive tract which play a crucial role during testicular development, epididymal sperm maturation, and sperm-oocyte fusion. The ADAMTS10 is a member of metalloproteinase family, the expression of which is important in sperm-oocyte interaction at the time of fertilization in mouse. In this species, the expression of ADAMTS10 has been demonstrated in testis, and on surface of spermatozoa from caput, corpus and cauda epididymis. Whether ADAMTS10 plays any role in male fertility of any of the domestic ruminant species including buffaloes is not known so far. Thus, a study was conducted to detect the expression of ADAMTS10 in testis, different parts of
the epididymis and ejaculated spermatozoa of water buffalo (Bubalus bubalis) using real-time PCR, Western blot, and indirect immunofluorescence techniques. The highest expression of ADAMTS10 transcript was observed in corpus epididymis, followed by cauda, caput and testis. The Western blot detection of ADAMTS10 using the heterologous antibody has demonstrated 31-, 44-, 50-, 65 kDa immune-reactive protein bands from testis and 82 kDa protein from both testes and corpus epididymis and a major 36 kDa protein from ejaculated spermatozoa. The indirect immunofluorescence study has demonstrated high fluorescence signal in post-acrosome and the middle piece region of the ejaculated spermatozoa. Thus the differential expression pattern of ADAMTS10 in different parts of male reproductive tract of buffalo indicates their role in sperm maturation process. However, specific role of ADAMTS10 in buffalo male reproduction can only be ascertained after further studies.
ICAR-NIANP
 
Date 2019-11-15T07:03:23Z
2019-11-15T07:03:23Z
2018-10-05
 
Type Research Paper
 
Identifier Gurupriya VS, Roy SC, Javvaji PK, Dhali A, Badami S, Rahim F, Divyashree BC, Panda AP. 2018. Expression of ADAMTS10 in male reproductive tract of buffaloes (Bubalus bubalis). Journal of Experimental Biology and Agricultural Sciences, 6:800-807.
2320 – 8694
http://krishi.icar.gov.in/jspui/handle/123456789/24761
 
Language English
 
Relation Not Available;
 
Publisher Horizon Publisher India