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Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes

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Title Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes
Not Available
 
Creator Soni RK
Gupta PSP
Nandi S
Mondal S
Ippala JR
Mor A
Mishra A
Tripathi SK
 
Subject CCND2,
CYP19A1
Estrus induction
Goat
mRNA expression
 
Description Not Available
The study was conducted by supplementing cupric chloride dihydrate to modulate the estradiol synthesis in granulosa cells with a hypothesis of possible use of copper to potentiate or partially replace the hormones for estrus induction / estrus synchronization in future studies. In present study copper at three doses (0.1, 0.5 and 1 mM level in culture medium) were tested to deserve see their effects on in vitro granulosa cell survival, estradiol synthesis and their associated genes of ovarian granulosa cells of goat.There was no effect of copper on the ovarian granulosa cell survival rate. There was a considerable increase in the estradiol level per ml culture medium basis by 6th day of in vitro culture with the second dose of copper i.e. 0.5 mM, but the increase was non-significant (P>0.05). There was no significant effect of copper on estradiol
synthesis when expressed on per 30000 cell basis. Effect of copper (0.1 mM and 0.5 mM) on the mRNA expression of genes of aromatase (CYP19A1) and cyclin D2 (CCND2) was estimated. Copper had significantly (P
ICAR-NIANP
 
Date 2019-11-15T08:50:55Z
2019-11-15T08:50:55Z
2018-03-30
 
Type Research Paper
 
Identifier Soni RK, Gupta PSP, Nandi S, Mondal S, Ippala JR, Mor A, Mishra A, Tripathi SK. 2018. Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes. Indian Journal of Animal Research, 52:652-657.
Print ISSN:0367-6722 / Online ISSN:0976-0555
http://krishi.icar.gov.in/jspui/handle/123456789/24784
 
Language English
 
Relation Not Available;
 
Publisher ARCC Journals