Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes
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Title |
Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes
Not Available |
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Creator |
Soni RK
Gupta PSP Nandi S Mondal S Ippala JR Mor A Mishra A Tripathi SK |
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Subject |
CCND2,
CYP19A1 Estrus induction Goat mRNA expression |
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Description |
Not Available
The study was conducted by supplementing cupric chloride dihydrate to modulate the estradiol synthesis in granulosa cells with a hypothesis of possible use of copper to potentiate or partially replace the hormones for estrus induction / estrus synchronization in future studies. In present study copper at three doses (0.1, 0.5 and 1 mM level in culture medium) were tested to deserve see their effects on in vitro granulosa cell survival, estradiol synthesis and their associated genes of ovarian granulosa cells of goat.There was no effect of copper on the ovarian granulosa cell survival rate. There was a considerable increase in the estradiol level per ml culture medium basis by 6th day of in vitro culture with the second dose of copper i.e. 0.5 mM, but the increase was non-significant (P>0.05). There was no significant effect of copper on estradiol synthesis when expressed on per 30000 cell basis. Effect of copper (0.1 mM and 0.5 mM) on the mRNA expression of genes of aromatase (CYP19A1) and cyclin D2 (CCND2) was estimated. Copper had significantly (P ICAR-NIANP |
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Date |
2019-11-15T08:50:55Z
2019-11-15T08:50:55Z 2018-03-30 |
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Type |
Research Paper
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Identifier |
Soni RK, Gupta PSP, Nandi S, Mondal S, Ippala JR, Mor A, Mishra A, Tripathi SK. 2018. Effect of in vitro copper supplementation on granulosa cell estradiol synthesis and associated genes. Indian Journal of Animal Research, 52:652-657.
Print ISSN:0367-6722 / Online ISSN:0976-0555 http://krishi.icar.gov.in/jspui/handle/123456789/24784 |
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Language |
English
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Relation |
Not Available;
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Publisher |
ARCC Journals
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