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Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm

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Title Isolation of a lytic bacteriophage against virulent Aeromonas hydrophila from an organized equine farm
veterinary type Culture Collection
National Research Centre on equines
National Institute of High Security Animal Diseases
Indian Veterinary Research Institute,
 
Creator Taruna Anand
Rajesh Kumar Vaid
Bidhan Ch. Bera
Jitender Singh
Sanjay Barua
Nitin Virmani
Rajukumar K.
Raj K. Singh
 
Subject Bacteriophage
 
Description Not Available
A bacteriophage (VTCCBPA6) against a pathogenic strain of Aeromonas hydrophila was isolated from the sewage of an organized equine breeding farm. On the basis of TEM analysis, phage belonged to family Myoviridae. PCR amplification and sequence analysis of gp23 gene (encoding for major capsid protein) revealed phylogenetic resemblance to T4 like virus genus. Protein profiling by SDS-PAGE also indicated its resemblance to T4 like phage group. However, the comparison of its gp23 gene sequence with previously reported phages showed similarity with T4-like phages infecting Enterobacteriaceae instead of Aeromonas spp. Thus, to our knowledge, this report points toward the fact that a novel/evolved phage might exist in equine environment against A. hydrophila, which can be potentially used as a biocontrol agent.
ICAR
 
Date 2019-12-05T05:03:16Z
2019-12-05T05:03:16Z
2016-04-01
 
Type Article
 
Identifier 2
1521-4028
http://krishi.icar.gov.in/jspui/handle/123456789/27690
 
Language English
 
Relation Not Available;
 
Publisher Wiley