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Transposon-based reprogramming to induced pluripotency

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Title Transposon-based reprogramming to induced pluripotency
Not Available
 
Creator Dharmendra Kumar
Thirumala R. Talluri
Taruna Anand
Wilfried A. Kues
 
Subject Induced pluripotent stem cells, Reprogramming, Transposition, Sleeping Beauty, PiggyBac, Stemness, Ontogenesis, Synthetic biology
 
Description Not Available
Induced pluripotent stem (iPS) cells
represent a recent innovation in the field of stem cells.
Commonly, iPS cells are generated by viral transduction
of core reprogramming genes, such as Oct4, Sox2, Klf4,
c-Myc, Nanog and Lin28. However, integrating viruses,
like retro- and lentiviral vectors, may cause insertional
mutagenesis and may increase the risk of tumor
formation. Therefore, alternative methods which avoid
these safety concerns are intensively investigated. Here,
we review the current status of transposon-based
methods to induce pluripotency. DNA transposons are
non-viral elements, which can be effectively integrated
into a genome by their corresponding transposase
enzyme. The advantages of transposon-based gene
transfer are their increased safety, their large cargo
capacity, their relatively simple design, and the
availability of hyper-active and mutated transposase
enzymes. For example, integration-deficient, excisioncompetent
transposase variants allow the complete
removal of the reprogramming transposon after
successful reprogramming to obtain transposon-free
reprogrammed cells. Transposon-based reprogramming
broaden the toolbox for iPS cell production and will
advance the establishment of safe, non-viral methods.
Not Available
 
Date 2019-12-05T05:04:52Z
2019-12-05T05:04:52Z
2015-12-01
 
Type Article
 
Identifier 2
0213-3911
http://krishi.icar.gov.in/jspui/handle/123456789/27692
 
Language English
 
Relation Not Available;
 
Publisher Histology and Histopathology