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Abundance of antibiotic resistance genes in environmental bacteriophages

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Title Abundance of antibiotic resistance genes in environmental bacteriophages
Not Available
 
Creator Taruna Anand,
Bidhan Ch. Bera,
Rajesh K. Vaid,
Sanjay Barua,
Thachamvally Riyesh,
Nitin Virmani,
Mubarik Hussain
Raj K. Singh
Bhupendra N. Tripathi
 
Subject Bacteriophage
 
Description Not Available
The ecosystem is continuously exposed to a wide variety of antimicrobials through waste
effluents, agricultural run - offs and animal - related and anthropogenic activities, which contribute
to the spread of antibiotic resistance genes (ARGs). The contamination of ecosystems with
ARGs may create increased opportunities for their transfer to naive microbes and eventually lead
to entry into the human food chain. Transduction is a significant mechanism of horizontal gene
transfer in natural environments, which has traditionally been underestimated as compared to
transformation. We explored the presence of ARGs in environmental bacteriophages in order to
recognize their contribution in the spread of ARGs in environmental settings. Bacteriophages
were isolated against environmental bacterial isolates, purified and bulk cultured. They were
characterized, and detection of ARG and intI genes including blaTEM, blaOXA - 2, intI1, intI2, intI3,
tetA and tetW was carried out by PCR. This study revealed the presence of various genes [tetA
(12.7 %), intI1 (10.9 %), intI2 (10.9 %), intI3 (9.1 %), tetW (9.1 %) and blaOXA - 2 (3.6 %)] and
blaTEM in a significantly higher proportion (30.9 %). blaSHV, blaOXA - 1, tetO, tetB, tetG, tetM and
tetS were not detected in any of the phages. Soil phages were the most versatile in terms of
ARG carriage. Also, the relative abundance of tetA differed significantly vis - a - vis source. The
phages from organized farms showed varied ARGs as compared to the unorganized sector,
although blaTEM ARG incidences did not differ significantly. The study reflects on the role of
phages in dissemination of ARGs in environmental reservoirs, which may provide an early
warning system for future clinically relevant resistance mechanisms.
ICAR
 
Date 2019-12-06T06:47:17Z
2019-12-06T06:47:17Z
2016-12-01
 
Type Research Paper
 
Identifier Not Available
0022-1317
http://krishi.icar.gov.in/jspui/handle/123456789/28405
 
Language English
 
Relation Not Available;
 
Publisher Microbiology Society, UK