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Cholesterol Loaded Cyclodextrin Increases cryopreservability of Marwari Stallion (Equus ferus caballus) Spermatozoa by Increasing Cholesterol to Phospholipid Ratio

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Title Cholesterol Loaded Cyclodextrin Increases cryopreservability of Marwari Stallion (Equus ferus caballus) Spermatozoa by Increasing Cholesterol to Phospholipid Ratio
 
Creator Pramod Kumar1*
J.S. Mehta1
S.K. Ravi2
Talluri T Rao3
G.N. Purohit1
Ashok Kumar Chaudhary1
 
Subject Marwari stallion
cholesterol loaded cyclodextrin (CLC)
cholesterol, phospholipid
cholesterol: phospholipid (C: P) ratio
 
Description Not Available
The study was conducted to investigate the effects of different levels of cholesterol loaded cyclodextrin (CLC) addition on cooled and frozen-thawed spermatozoa of Marwari stallion. A total of 48 ejaculates were collected from six adult Marwari stallions (8 ejaculates from each stallion) aged between 4 to 7 years. Immediately after collection semen sample was macroscopically evaluated and filtered into a warm, graduated measuring bottle to get gel free semen. The level of cholesterol (C) and phospholipid (P) in fresh spermatozoa were measured using ELISA. The semen sample was divided in to five equal aliquots (T0, T1, T2, T3 and T4). Primary extender containing different concentrations of CLC was added to each aliquot (0, 1, 1.5, 2 and 3 mg/ml CLC in T0, T1, T2, T3 and T4, respectively). All the aliquots were incubated for 15 minutes at 37°C for incorporation of CLC in sperm plasma membrane and then cryopreserved. Level of C and P in spermatozoa was also evaluated at pre-freeze and post-thaw stages. The mean C, P and C: P ratio in fresh sperm was 15.36±0.47 μg/100 × 106 sperm cells, 46.21±1.27 μg/100 × 106 sperm cells and 0.33±0.071, respectively. The mean C content and C: P ratio were significantly higher (P
ICAR
 
Date 2019-12-05T11:00:21Z
2019-12-05T11:00:21Z
2019-03-30
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28159
 
Language English
 
Relation journal of Animal Research: v.9 n.2, p. 227-235. April 2019;
 
Publisher New Delhi Publishers