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Chicken Mx gene polymorphisms in Indian native chicken breeds and White Leghorn by real time multiplex allele specific PCR

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Title Chicken Mx gene polymorphisms in Indian native chicken breeds and White Leghorn by real time multiplex allele specific PCR
Not Available
 
Creator Kannaki T. Ramasamy*, M. R. Reddy, K. S. Raja Ravindra and R. N. Chatterjee
 
Subject Chicken
Mx Gene polymorphism
Real time PCR
 
Description Not Available
Chicken Mx protein confers resistance against viral infections including avian influenza virus. Mx protein is one of the
strong antiviral proteins induced by interferon system. Different alleles of chicken Mx gene have been shown to vary in
their antiviral activity against influenza virus. A non-synonymous mutation at position 2032 of chicken Mx gene responsible
for variation of amino acid (Ser to Asn) in Mx protein is responsible for negative to positive antiviral activity of Mx protein.
In the present study it was analyzed that SNP at G2032A in chicken Mx gene in two Indian native chicken breeds, Aseel,
Kadaknath and compared with White Leghorn layer. A total of 90 samples, including 50 of White Leghorn and 20 each from
native chicken breed were screened for SNP at position 2032 of Mx gene by using real time multiplex allele specific PCR. The
resistant allele (A) frequency is higher than susceptible allele in all three chicken breeds investigated. Among the breeds,
the resistant allele frequency (A) is higher in White Leghorn (0.95) followed by Aseel (0.75) and Kadaknath (0.625).
Homozygous to resistant allele (AA) is the major genotype in White Leghorn whereas heterozygous to both alleles is the
major genotype in Kadaknath. Variations in the gene frequency might have emerged from varying degree of selection
pressure the different breeds had undergone. In future, the resistant genotype could be selected in modern breeding
programs to improve the genetic status for disease resistance against viral infections.
Not Available
 
Date 2019-12-05T05:04:56Z
2019-12-05T05:04:56Z
2017-11-13
 
Type Article
 
Identifier Not Available
DOI: 10.18805/ijar.B-3285
http://krishi.icar.gov.in/jspui/handle/123456789/27693
 
Language English
 
Relation Not Available;
 
Publisher Agriculture Research communication centre