Record Details

Molecular characterization and computational structure prediction of activin receptor type IIB in Aseel and broiler chicken

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular characterization and computational structure prediction of activin receptor type IIB in Aseel and broiler chicken
Not Available
 
Creator Guru Vishnu, P., Bhattacharya, T.K., Bhushan, B., Kumar, P., Chatterjee, R.N., Paswan, C., Rajendra Prasad, A., Divya, D. and Dushyanth, K.
 
Subject Characterization ACTRIIB Three dimensional structure Ramachandran plot Hydrogen bond plot Contact map
 
Description Not Available
The present study was formulated to characterize and comprehend the molecular structural characteristics of ACTRIIB receptor in Aseel and control broiler (CB) populations. The full length coding sequence (1539 bp) of the receptor was amplified, cloned, sequenced and analyzed using bioinformatic tools. The physico chemical properties of protein and structural features like secondary structure, solvent accessibility and disorder regions were computed. The 3D structure was predicted by I-TASSER and evaluated by Ramachandran Plot and tools under Structural Analysis and Verification Server. The nucleotides differences between CB and Aseel were c. [156G > A; 210 T > C; 493C > T; c.520G > C; 665A > C; 686G > A; 937C > G; 1011A > C; 1130A > G; 1208 T > A; 1326 T > C; 1433 T > C]. The amino acid substitutions between CB and Aseel were p. [(Pro165Ser; Glu174Gln; Gln222Pro; Ser229Asn; His313Asp; Gln377Arg; Val403Asp; and Ile478Thr)]. While, the silent changes includes p. [(Lys53=; Glu71=; Leu337=; Asp442=)]. The molecular weight of mature protein was predicted to be 55.51 kDa and 57.80 kDa in Aseel and CB, respectively. The higher rank 3D model had a C-score of −1.60 in Aseel and − 1.41 in CB, while the estimated TM-score (0.54 ± 0.14) and RMSD (5.8 ± 1.2 Å) were found to be similar in Aseel and CB. Among the 512 residues, >90% were in favored region, 4.7% in allowed region and
Not Available
 
Date 2019-12-05T04:57:21Z
2019-12-05T04:57:21Z
2019-08-27
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/27681
 
Language English
 
Relation Not Available;
 
Publisher Not Available