Molecular characterization and computational structure prediction of activin receptor type IIB in Aseel and broiler chicken
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Molecular characterization and computational structure prediction of activin receptor type IIB in Aseel and broiler chicken
Not Available |
|
Creator |
Guru Vishnu, P., Bhattacharya, T.K., Bhushan, B., Kumar, P., Chatterjee, R.N., Paswan, C., Rajendra Prasad, A., Divya, D. and Dushyanth, K.
|
|
Subject |
Characterization ACTRIIB Three dimensional structure Ramachandran plot Hydrogen bond plot Contact map
|
|
Description |
Not Available
The present study was formulated to characterize and comprehend the molecular structural characteristics of ACTRIIB receptor in Aseel and control broiler (CB) populations. The full length coding sequence (1539 bp) of the receptor was amplified, cloned, sequenced and analyzed using bioinformatic tools. The physico chemical properties of protein and structural features like secondary structure, solvent accessibility and disorder regions were computed. The 3D structure was predicted by I-TASSER and evaluated by Ramachandran Plot and tools under Structural Analysis and Verification Server. The nucleotides differences between CB and Aseel were c. [156G > A; 210 T > C; 493C > T; c.520G > C; 665A > C; 686G > A; 937C > G; 1011A > C; 1130A > G; 1208 T > A; 1326 T > C; 1433 T > C]. The amino acid substitutions between CB and Aseel were p. [(Pro165Ser; Glu174Gln; Gln222Pro; Ser229Asn; His313Asp; Gln377Arg; Val403Asp; and Ile478Thr)]. While, the silent changes includes p. [(Lys53=; Glu71=; Leu337=; Asp442=)]. The molecular weight of mature protein was predicted to be 55.51 kDa and 57.80 kDa in Aseel and CB, respectively. The higher rank 3D model had a C-score of −1.60 in Aseel and − 1.41 in CB, while the estimated TM-score (0.54 ± 0.14) and RMSD (5.8 ± 1.2 Å) were found to be similar in Aseel and CB. Among the 512 residues, >90% were in favored region, 4.7% in allowed region and Not Available |
|
Date |
2019-12-05T04:57:21Z
2019-12-05T04:57:21Z 2019-08-27 |
|
Type |
Research Paper
|
|
Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/27681 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Not Available
|
|