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In silico prediction of short hairpin and in vitro silencing of activin receptor type IIB in chicken embryo fibroblasts by RNA interference

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Title In silico prediction of short hairpin and in vitro silencing of activin receptor type IIB in chicken embryo fibroblasts by RNA interference
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Creator Guru Vishnu, P., Bhattacharya, T.K., Bhushan, B., Kumar, P., Chatterjee, R.N., Paswan, C., Dushyanth, K., Divya, D. and Rajendra Prasad, A.
 
Subject Muscle growth ACTRIIB Secondary structures Thermodynamic parameters Gene expression Gene silencing
 
Description Not Available
Gene silencing by RNA interference is extensively used reverse genetic approach to analyse the implications of any gene in mammalian systems. The silencing of the Activin type IIB receptor belonging to transforming growth factor beta superfamily has demonstrated increase in muscle growth in many species. We designed five short hairpin RNA constructs targeting coding region of chicken ACTRIIB. All the shRNAs were transfected into chicken embryo fibroblast cells and evaluated their silencing efficiency by real time PCR and western blotting. Initially the computational analysis of target region and shRNA constructs was undertaken to predict sequence based features (secondary structures, GC% and H-b index) and thermodynamic features (ΔGoverall, ΔGduplex, ΔGbreak−target, ΔGintra−oligomer, ΔGinter−oligomer and ΔΔGends). We determined that all these predicted features were associated with shRNA efficacy. The invitro analysis of shRNA constructs exhibited significant (P 
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Date 2019-12-05T04:59:56Z
2019-12-05T04:59:56Z
2019-06-01
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/27683
 
Language English
 
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Publisher Not Available