Record Details

Effect of thermal manipulation during embryogenesis on liver heat shock protein expression in chronic heat stressed colored broiler chickens.

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Effect of thermal manipulation during embryogenesis on liver heat shock protein expression in chronic heat stressed colored broiler chickens.
Not Available
 
Creator A.Vinoth, T.Thirunal Sundari, J.A. Therian, M.Shanmugam and U.Rajkumar
 
Subject Broiler; Heat Shock Protein; Naked neck; Thermal manipulation
 
Description Not Available
Thermal manipulation during embryogenesis has been shown to improve thermo tolerance in broilers. Heat shock proteins are a family of proteins produced in response to variety of stress and protect cells from damage. The aim of this study was to evaluate the effect of thermal manipulation (TM) during embryogenesis on HSP gene and protein expression in the embryos and in chronic heat stressed 42nd day old chicks. On 15th day of incubation, fertile eggs from two breeds-Naked neck (NN) and Punjab Broiler-2 (PB-2) were randomly divided in to two groups, namely Control (C) eggs were incubated under standard incubation conditions and Thermal Conditioning (TC) eggs were exposed to higher incubation temperature (40.5°C) for 3h on 15th, 16th and 17th day of incubation. The chicks so obtained from each group were further subdivided and reared from 15th-42nd day as normal (N; 25±1°C, 70% RH) and heat exposed (HE; 35±1°C, 50% RH) resulting in four treatment groups (CN, CHE, TCN and TCHE). Embryos of two groups (C and TC) on 17th day and birds from four treatment groups on 42nd day were sacrificed. Liver was collected for analysis of gene expression by real-time PCR and protein expression by Western blot of Heat Shock Proteins (HSP 90 alpha, HSP 90 beta, HSP 70, HSP 60, HSP 27 and ubiquitin). The plasma collected on 42nd day was analyzed for biochemical parameters. Thermal challenging of embryos of both the breeds caused significant (P≤0.05) increase in all the HSPs gene and protein expression. The TCHE chicks had significantly (P≤0.05) lower HSPs gene and protein expressions and oxidative stress compared to CHE groups in both NN and PB-2. Based on these findings it can be concluded that TM during incubation provides adaptation to broiler chicks during chronic heat stress.
ICAR-NICRA
 
Date 2019-12-05T08:32:46Z
2019-12-05T08:32:46Z
2015-10-01
 
Type Article
 
Identifier A.Vinoth, T.Thirunal Sundari, J.A. Therian, M.Shanmugam and U.Rajkumar. 2015.Effect of thermal manipulation during embryogenesis on liver heat shock protein expression in chronic heat stressed colored broiler chickens. J Therm Biol. 2015 Oct;53:162-71
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/27974
 
Language English
 
Relation Not Available;
 
Publisher Elsevier