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Molecular characterization of internal transcribed spacer 1 (ITS 1) region of different Trypanosoma evansi isolates of India

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Title Molecular characterization of internal transcribed spacer 1 (ITS 1) region of different Trypanosoma evansi isolates of India
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Creator Souti Prasad Sarkhel • Surender Kumar Gupta • Jyoti Kaushik • Jarnail Singh • Deepak Kumar Gaur • Sanjay Kumar • Rajender Kumar
 
Subject Molecular characterization Internal transcribed spacer 1 ITS 1 Trypanosoma evansi
 
Description Not Available
Six Trypanosoma evansi isolates collected from
ponies (PH1 and PK6), camel (CB2), donkeys (DJ3 and
DH4) and cattle (CK5) from Haryana, Rajasthan, Uttar
Pradesh and Gujarat states of India were used for molecular
characterization of internal transcribed spacer 1 (ITS 1).
The DNA was isolated from purified trypanosomes of these
six isolates after propagation in mice model. ITS1-PCR of
purified parasite DNA yielded an amplification product
approximately 540 bp in size. Nucleotide sequence of ITS1
gene of CB2 isolate had 530 bp while CK5, DH4, DJ3, and
PH1 isolates had 532 bp, whereas, PK6 isolates had 533 bp
size. Blast data of the Indian isolates revealed 99 %
homology with other available sequences of T. evansi.
Multiple alignment of nucleotide sequence of ITS1 gene
variants from Indian T. evansi isolates with selected
homologous sequences from GenBank revealed that
nucleotide substitution mostly occurred at the position of
101–103, 218–223, 243–244, 301–396 and 470–480. The
isolates PH1, CK5, DH4 and DJ3 were found more associated
with T. evansi isolates from the Philippines, Thailand,
Iran, Egypt and China, whereas, PK6 and CB2
isolates were related to each other and were phylogenetically
distant from rest of the Indian isolates used in this
study. Based on the ITS1 rDNA sequence, the Neighbour–
Joining consensus tree indicated clear evidence of
existence of genetic diversity among T. evansi isolates
from India.
Not Available
 
Date 2019-12-06T06:46:36Z
2019-12-06T06:46:36Z
2017-06-01
 
Type Book
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/28401
 
Language English
 
Relation Not Available;
 
Publisher Springer