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Antigenic characterization of 52–55 kDa protein isolated from Trypanosoma evansi and its application in detection of equine trypanosomosis

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Title Antigenic characterization of 52–55 kDa protein isolated from Trypanosoma evansi and its application in detection of equine trypanosomosis
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Creator S.C. Yadav, Ritesh Kumar, Jaideep Kumar, Meetali Singh, B.C. Bera, Rajender Kumar, U. Tatu, Kanika Tehri
 
Subject Equine trypanosomosis, Trypanosoma evansi, 52–55 kDa cluster Immuno-dominant antigen Diagnosis Proteomic analysis
 
Description Not Available
Trypanosoma evansi is a haemo-protozoan parasite responsible for the disease surra, an economically important
disease of wide range of domestic and wild animals. The present diagnostic methods using soluble antigens have
inherent problems like lack of standardized and reproducible antigens, as well as ethical issues. This entails
further efforts for search of defined antigenic molecules with satisfying sensitivity and specificity for sero-epidemiology
of trypanosomosis. In present investigation, we have identified and purified 52–55 kDa immunodominant
protein cluster in molecular mass ranges by preparatory SDS-PAGE methods from T. evansi proteome.
The purified protein was further characterized by hyper immune serum raised in rabbits and also further
evaluated for its immunodiagnostic potential using experimentally infected horse serum samples by different
immunological tests. The immunoblot, ELISA and dot blot assay using purified cluster in infected pooled serum
samples showed detection of infection early as 10th days post infection till termination of experiment. The
observations revealed that purified cluster is expressed not only at early stage but also persisted and detected
throughout course of infection. Further, whole cell lysate antigen separated out and detected 141 spots by 2-D
gel electrophoresis. The isoelectric focussing (PI) of 52–55 kDa was determined in pH range between 6.9 and 7.5
along with two other cluster of proteins recognised by immune sera of ponies infected with T. evansi. MS/MS
analysis of the purified protein cluster identified five proteins i.e. pyruvate kinase 1, beta tubulin, paraflagellar
rod protein, alanine aminotransferase and variable surface glycoprotein showing homology to protein present in
Trypanosome database. These identified proteins may be useful for development of vaccines and diagnostic
targets against animal trypanosomosis.
Not Available
 
Date 2019-12-06T07:24:33Z
2019-12-06T07:24:33Z
2017-07-13
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28410
 
Language English
 
Relation Not Available;
 
Publisher Elsevier