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Differentiation of induced pluripotent stem cells to lentoid bodies expressing a lens cell-specific fluorescent reporter.

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Title Differentiation of induced pluripotent stem cells to lentoid bodies expressing a lens cell-specific fluorescent reporter.
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Creator Taruna Anand
Talluri TR
Dharmendra Kumar
Weibke Garrels
Mukherjee A
Debowski K
Behr R
Wilfried Kues
 
Subject iPS cells, lentoid bodies, murine
 
Description Not Available
Curative approaches for eye cataracts and other eye abnormalities, such as myopia and hyperopia currently suffer from a lack of appropriate models. Here, we present a new approach for in vitro growth of lentoid bodies from induced pluripotent stem (iPS) cells as a tool for ophthalmological research. We generated a transgenic mouse line with lens-specific expression of a fluorescent reporter driven by the alphaA crystallin promoter. Fetal fibroblasts were isolated from transgenic fetuses, reprogrammed to iPS cells, and differentiated to lentoid bodies exploiting the specific fluorescence of the lens cell-specific reporter. The employment of cell type-specific reporters for establishing and optimizing differentiation in vitro seems to be an efficient and generally applicable approach for developing differentiation protocols for desired cell populations.
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Date 2019-12-06T09:13:01Z
2019-12-06T09:13:01Z
2016-06-20
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28446
 
Language English
 
Relation Not Available;
 
Publisher ‎Public Library of Science