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A comparative evaluation of parasitological, serological and DNA amplification methods for diagnosis of natural Trypanosoma evansi infection in camels

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Title A comparative evaluation of parasitological, serological and DNA amplification methods for diagnosis of natural Trypanosoma evansi infection in camels
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Creator Narender Singh, K.M.L. Pathak, Rajender Kumar
 
Subject Trypanosoma evansi; Camels; Diagnosis-protozoa; Ag-ELISA; PCR
 
Description Not Available
A representative number of 217 camels (Camelus dromedarius) from different areas of western
Rajasthan State, India, were examined from July 2002 to May 2003 for Trypanosoma evansi
infection. The tests used were parasitological (wet blood film, WBF; stained thin blood smear, TBS),
immunodiagnostic (double antibody sandwich enzyme linked immunosorbent assay for antigen
detection, Ag-ELISA), and DNA amplification by polymerase chain reaction (PCR). These techniques
were compared and the best efficiency was found for the last named (PCR). A prevalence of T.
evansi infection was detected in 17.05, 9.67, 4.60 and 4.14% by PCR, Ag-ELISA, TBS and WBF
with a sensitivity of 100, 56.75, 27.02 and 24.32%, respectively. PCR revealed a specific 227 bp band
in positive samples. The intensity of PCR bands was variable in different test samples depending
upon the level of infection in the test samples. The history of intermittent fever, emaciation, oedema,
poor body condition significantly correlated with positive serological status in ELISA as well as
trypanosome DNA detection by PCR
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Date 2019-12-06T09:38:56Z
2019-12-06T09:38:56Z
2004-08-03
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28461
 
Language English
 
Relation Not Available;
 
Publisher Elsevier