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Pathology of Equine Influenza virus (H3N8) in Murine Model

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Title Pathology of Equine Influenza virus (H3N8) in Murine Model
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Creator Not Available
 
Subject equine influenza
 
Description Not Available
Equine influenza viruses (EIV)—H3N8 continue to circulate in equine population throughout
the world. They evolve by the process of antigenic drift that leads to substantial change in
the antigenicity of the virus, thereby necessitating substitution of virus strain in the vaccines.
This requires frequent testing of the new vaccines in the in vivo system; however, lack of an
appropriate laboratory animal challenge model for testing protective efficacy of equine influenza
vaccine candidates hinders the screening of new vaccines and other therapeutic
approaches. In the present investigation, BALB/c mouse were explored for suitability for
conducting pathogenecity studies for EIV. The BALB/c mice were inoculated intranasally@
2×106.24 EID50 with EIV (H3N8) belonging to Clade 2 of Florida sublineage and monitored
for setting up of infection and associated parameters. All mice inoculated with EIV exhibited
clinical signs viz. loss in body weights, lethargy, dyspnea, etc, between 3 and 5 days which
commensurate with lesions observed in the respiratory tract including rhinitis, tracheitis,
bronchitis, bronchiolitis, alveolitis and diffuse interstitial pneumonia. Transmission electron
microscopy, immunohistochemistry, virus quantification through titration and qRT-PCR
demonstrated active viral infection in the upper and lower respiratory tract. Serology
revealed rise in serum lactate dehydrogenase levels along with sero-conversion. The pattern
of disease progression, pathological lesions and virus recovery from nasal washings
and lungs in the present investigations in mice were comparable to natural and experimental
EIV infection in equines. The findings establish BALB/c mice as small animal model for
studying EIV (H3N8) infection and will have immense potential for dissecting viral pathogenesis,
vaccine efficacy studies, preliminary screening of vaccine candidates and antiviral
therapeutics against EIV.
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Date 2019-12-07T09:27:54Z
2019-12-07T09:27:54Z
2015-11-20
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28687
 
Language English
 
Relation Not Available;
 
Publisher Not Available