Record Details

Genetic Analysis of the Neuraminidase (NA) Gene of Equine Influenza Virus (H3N8) from Epizootic of 2008–2009 in India

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Genetic Analysis of the Neuraminidase (NA) Gene of Equine Influenza Virus (H3N8) from Epizootic of 2008–2009 in India
Not Available
 
Creator B.C.Bera
Nitin Virmani
 
Subject equine influenza
 
Description Not Available
The neuraminidase (NA) gene sequences of four
Indian equine influenza viruses (EIVs) isolated from epizootic
in 2008 and 2009were analyzed. The phylogenetic relationship
and selection pressure of NA genes were established in comparison
to other EIVs circulating worldwide along with the
domains and motifs of the encoded protein to find out the significance
of mutational changes. Among Indian isolates, two
amino acid (aa) changes each in Mysore/12/08 (Asn67Tyr &
Asp396Gly), Gopeshwar/1/09 (Ile49Val & Asp396Gly), and
Uttarkashi/1/09 (Ile49Val & Asp396Gly) isolates were
observed in respect to Jammu-Katra/06/08 isolate. Amino acid
(aa) sequence analysis also revealed five consistent aa residue
changes viz, Gly/Arg40Glu, Tyr66His, Val191Ile, Val209Ile
and Asp235Asn in Asian including Indian isolates, Spain/07
and Spain/09 isolates in comparison to other EIVs circulating
worldwide. The topology of the phylogenetic tree revealed that
the Indian, Chinese, Mongolian and Kazakhstan isolates together
formed a subgroupwith Yokohama/10 isolate. Spain/07&
Spain/09 isolates showed closest clusteringwithAsian isolates.
This indicates that non-synonymousmutations in Asian isolates
with temporal pattern originating from Spain/07, led to the
subgroup of the Asian isolates within Florida clade 2 sublineage.
The analysis of the predicted secondary structure has not
shown any significant difference in the NA proteins of all
Indian isolates. Fixed-effects likelihood (FEL) analysis of the
selection pressure revealed three codons (43, 355 & 434)
under positive selection pressure. The overall evolutionary
changes (x value) of 3.4 indicatesNA gene to be under strong
selection pressure. Further, seven putative N-glycosylation
siteswere observed in theNAprotein.Themapping of specific
aa changes, theirmutational and functional analysis need to be
carried out to ascertain their role in pathogenecity of the virus.
Not Available
 
Date 2019-12-07T09:28:26Z
2019-12-07T09:28:26Z
2013-05-24
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28689
 
Language English
 
Relation Not Available;
 
Publisher Not Available