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Immunogenicity and protective efficacy of inactivated equine influenza (H3N8) virus vaccine in murine model

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Title Immunogenicity and protective efficacy of inactivated equine influenza (H3N8) virus vaccine in murine model
Not Available
 
Creator Pavul raj
Nitin Virmani
 
Subject equine influenza
 
Description Not Available
Equine influenza viruses (EIVs) are responsible for acute contagious respiratory infection in equines and the
disease remains a major threat for equine population throughout the world despite vaccination strategies in
place. The present study was aimed to assess the suitability of BALB/c mice as a potential small animal model for
preliminary screening of EI vaccine candidates. For this, we evaluated the immunogenicity and protective efficacy
of an inactivated EIV (H3N8) vaccine in BALB/c mouse model after challenge with homologous H3N8
virus (Clade 2 virus, Florida sublineage) through serology, clinical signs, gross and histopathology lesions with
grading, immunohistochemistry and virus quantification. Serological responses in immunized mice were evaluated
by haemagglutination inhibition assay (HAI) and antibodies were subtyped by ELISA. The vaccine induced
optimum protective antibody titre on 49 dpi along with balanced Th1/Th2 responses. Immunized mice were well
protected against EIV challenge as evident by significant rise in serum antibody titre which concurred with mild
clinical signs, early recovery, lower gross and histopathological lesions score, less severe intensity of viral antigen
distribution, restricted virus replication in respiratory tract and less virus detection in nasal washes for
short duration. The duration of the viral load was also lower and only for brief period as compared to unvaccinated
challenged mice. In conclusion, induction of H3N8 specific antibody response and protection against
H3N8 challenge proves that egg grown inactivated H3N8 whole virus vaccine would provide an effective intercession
against H3N8 virus. In addition, BALB/c mouse can serve as an attractive tool for adjudging protective
efficacy of vaccine candidates prior to final testing in equines.
Not Available
 
Date 2019-12-07T09:28:48Z
2019-12-07T09:28:48Z
2017-08-18
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/28691
 
Language English
 
Relation Not Available;
 
Publisher Not Available