Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV).
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Title |
Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV).
Not Available |
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Creator |
Kalaiyarasu S
Mishra N Rajukumar K Behera SP Jhade SK Singh VP |
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Subject |
Border disease; Persistent infection; Hair follicle; Real time RT-PCR; Virus isolation
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Description |
Not Available
The aim of this study was to develop an improved border disease virus (BDV) specific real time RT-PCR and to evaluate its performance on manually plucked hairs from sheep persistently infected with BDV that may act as a non-invasive alternate sample. The BDV real time RT-PCR assay reported here showed a high analytical sensitivity (100.6 TCID50/ml), specificity (no reactivity with BVDV-1, BVDV-2, HoBi-like pestivirus and CSFV) and reproducibility. When the assay was validated on 210 samples from BDV-infected and uninfected sheep, it showed a 100% diagnostic sensitivity and specificity with virus isolation. Further evaluation of the assay on manually plucked hair follicles from ear (mid-lateral, mid-medial) and tail tip from sheep persistently infected with BDV showed that a minimum of 20 hair follicles need to be tested for correct diagnosis of BDV. The BDV load was comparatively higher in hairs from mid-medial ear than those from other tested locations. Evaluation on other samples from PI sheep demonstrated that the test performance was similar to that of pestivirus generic real-time RT-PCR, but improved than the currently available BDV specific real-time RT-PCR. Although more number of PI animals need to be evaluated, the results of the study showed that manually plucked hairs from mid-medial ear pinna is a suitable alternative sample in real-time RT-PCR for detection of BDV persistently infected sheep. Use of the non-invasive ear hair samples and the improved BDV specific real-time RT-PCR reported here may be useful for BDV surveillance in several sheep rearing countries. Not Available |
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Date |
2020-01-08T06:27:48Z
2020-01-08T06:27:48Z 2019-04-03 |
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Type |
Article
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Identifier |
Kalaiyarasu S, Mishra N, Rajukumar K, Behera SP, Jhade SK, Singh VP (2019) Development and evaluation of real-time RT-PCR using ear hair for specific detection of sheep persistently infected with border disease virus (BDV). Journal of Virological methods. 269:55-63.
0166-0934 http://krishi.icar.gov.in/jspui/handle/123456789/29387 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Elsevier B.V.
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