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Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus

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Title Characterization of GM-CSF-inhibitory factor and Uracil DNA glycosylase encoding genes from camel pseudocowpoxvirus
Not Available
 
Creator Nagarajan G
Swami SK
Dahiya SS
Dahiya SS
Narnaware SD
Mehta SC
Singh PK
Singh R
Tuteja FC
Patil NV
 
Subject Contagious ecthyma
Dromedary camel
GM-CSF-inhibitory factor
Parapoxviruses
Uracil DNA glycosylase
 
Description Not Available
The present study describes the PCR amplification of GM-CSF-inhibitory factor (GIF) and Uracil DNA glycosylase (UDG) encoding genes of pseudocowpoxvirus (PCPV) from the Indian Dromedaries (Camelus dromedarius) infected with contagious ecthyma using the primers based on the corresponding gene sequences of human PCPV and reindeer PCPV, respectively. The length of GIF gene of PCPV obtained from camel is 795 bp and due to the addition of one cytosine residue at position 374 and one adenine residue at position 516, the open reading frame (ORF) got altered, resulting in the production of truncated polypeptide. The ORF of UDG encoding gene of camel PCPV is 696 bp encoding a polypeptide of 26.0 kDa. Comparison of amino acid sequence homologies of GIF and UDG of camel PCPV revealed that the camel PCPV is closer to ORFV and PCPV (reference stains of both human and reindeer), respectively.
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Date 2020-02-12T05:01:54Z
2020-02-12T05:01:54Z
2015-01-01
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/31808
 
Language English
 
Relation Not Available;
 
Publisher Not Available