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Successful cloning of a superior buffalo bull

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Title Successful cloning of a superior buffalo bull
Not Available
 
Creator Naresh L. Selokar
Papori Sharma
Monika Saini
Suman Sheoran
Rasika Rajendran
Dharmendra Kumar
Rakesh Kumar Sharma
Rajender K. Motiani
Pradeep Kumar
A Jerome
Sudhir Khanna
Prem SinghYadav
 
Subject Buffalo
Cloning
Bull
 
Description Not Available
Somatic cell nuclear transfer (SCNT) technology provides an opportunity to multiply superior animals
that could speed up dissemination of favorable genes into the population. In the present study, we
attempted to reproduce a superior breeding bull of Murrah buffalo, the best dairy breed of buffalo,
using donor cells that were established from tail-skin biopsy and seminal plasma. We studied several
parameters such as cell cycle stages, histone modifcations (H3K9ac and H3K27me3) and expression of
developmental genes in donor cells to determine their SCNT reprogramming potentials. We successfully
produced the cloned bull from an embryo that was produced from the skin-derived cell. Growth,
blood hematology, plasma biochemistries, and reproductive organs of the produced cloned bull were
found normal. Subsequently, the bull was employed for semen production. Semen parameters such
as CASA (Computer Assisted Semen Analysis) variables and in vitro fertilizing ability of sperms of the
cloned bull were found similar to non-cloned bulls, including the donor bull. At present, we have 12 live
healthy progenies that were produced using artificial insemination of frozen semen of the cloned bull,
which indicate that the cloned bull is fertile and can be utilized in the buffalo breeding schemes. Taken
together, we demonstrate that SCNT can be used to reproduce superior buffalo bulls.
Not Available
 
Date 2020-07-08T10:45:29Z
2020-07-08T10:45:29Z
2019-08-06
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/38052
 
Language English
 
Relation Not Available;
 
Publisher Springer