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Genetic diversity of Cahi DRB and DQB genes of caprine MHC class II in Sirohi goat

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Title Genetic diversity of Cahi DRB and DQB genes of caprine MHC class II in Sirohi goat
Not Available
 
Creator G. R. GOWANE
NAJIF AKRAM
S. S. MISRA
VED PRAKASH
ARUN KUMAR
 
Subject Cahi-DRB
Cahi-DQB1
major histocompatibility complex
vaccine response
 
Description Not Available
The objective of this study was to assess the genetic diversity of the Sirohi goat for DRB and DQB1 loci, and to study their association with antibody response induced by the Peste des petits ruminants (PPR) vaccine. A total of 360 Sirohi kids were studied using single stranded confirmation polymorphism (SSCP) followed by polymerase chain reaction sequence-based typing (PCR-SBT) for DRB and DQB1 diversities. The competitive enzyme-linked immuno-sorbent assay (C-ELISA) was used to evaluate immune response post-PPR vaccination. Study revealed rich diversity of major histocompatibility complex (MHC) region in goat. A total of 18 DRB and 15 DQB1 alleles were obtained which were new. Alleles DRB*0104 and DQB1*0101 were the most common. The approach of SSCP combined with PCR-SBT reflects cost-effective and most powerful approach to decipher the genetic diversity in complex MHC region. Study revealed variation in DRB and DQB1 genes in Sirohi flock along with high Wu–Kabat index. A total of 16 of the 89 amino acid residue sites in DRB and 19 of 86 residue sites in DQB1 had more than three amino acid substitutions. Positive evolutionary selection was evident in Sirohi for MHC region. Nonsignificant association of DRB and DQB1 genotypes with PPR virus (PPRV) vaccine response revealed complexity of the phenotype and importance of other factors for vaccine response. Rich diversity of DRB and DQB1 genes reflects the fitness of the population and importance of this locus for future selection programmes.
Not Available
 
Date 2020-03-03T04:56:11Z
2020-03-03T04:56:11Z
2018-06-01
 
Type Article
 
Identifier Not Available
0022-1333
http://krishi.icar.gov.in/jspui/handle/123456789/33509
 
Language English
 
Relation Not Available;
 
Publisher Not Available