Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls.
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Title |
Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls.
Not Available |
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Creator |
SINGH R
JUNGHARE V HAZRA S SINGH U SENGAR GS RAJA TV KUMAR S TYAGI S DAS AK KUMAR A KORINGA P JAKHESARA S JOSHI CJ DEB R |
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Subject |
Frieswal Spermatozoa Transcriptome RNA deep sequencing
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Description |
Not Available
Bull spermatozoa contain different functional genes and many of them plays important roles in differentstages of spermatogenesis, spermatozoa kinetics, fertilization as well as embryonic development. RNAdeep sequencing is one of the preferred tools for absolute quantification of messenger RNA. The intentionof the current study was to investigate the abundance of spermatozoal transcripts in categorized Frieswal(Holstein-Friesian X Sahiwal) crossbred bull semen through RNA deep sequencing. A total 1546561 and1019308 numbers of reads were identified among good and poor quality bull spermatozoa based on theirconception rate. Post mapping withBos taurusreference genome identified 1,321,236 and 842,022number of transcripts among good and poor quality RNA libraries, respectively. However, a total numberof 3510 and 6759 functional transcripts were identified among good and poor quality bull spermatozoa,respectively. Most of the identified transcripts were related to spermatozoa functions, embryonicdevelopment and other functional aspects of fertilization. Wet laboratory validation of the topfiveselected transcripts (AKAP4, PRM1, ATP2B4, TRIM71 and SLC9B2) illustrated the significant (p Science and Engineering Research Board |
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Date |
2020-04-20T07:56:39Z
2020-04-20T07:56:39Z 2019-01-01 |
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Type |
Research Paper
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Identifier |
THERIOGENOLOGY, 129:130-145
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/34907 |
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Language |
English
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Relation |
Not Available;
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Publisher |
THERIOGENOLOGY
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