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Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls.

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Title Database on spermatozoa transcriptogram of catagorised Frieswal crossbred (Holstein Friesian X Sahiwal) bulls.
Not Available
 
Creator SINGH R
JUNGHARE V
HAZRA S
SINGH U
SENGAR GS
RAJA TV
KUMAR S
TYAGI S
DAS AK
KUMAR A
KORINGA P
JAKHESARA S
JOSHI CJ
DEB R
 
Subject Frieswal Spermatozoa Transcriptome RNA deep sequencing
 
Description Not Available
Bull spermatozoa contain different functional genes and many of them plays important roles in differentstages of spermatogenesis, spermatozoa kinetics, fertilization as well as embryonic development. RNAdeep sequencing is one of the preferred tools for absolute quantification of messenger RNA. The intentionof the current study was to investigate the abundance of spermatozoal transcripts in categorized Frieswal(Holstein-Friesian X Sahiwal) crossbred bull semen through RNA deep sequencing. A total 1546561 and1019308 numbers of reads were identified among good and poor quality bull spermatozoa based on theirconception rate. Post mapping withBos taurusreference genome identified 1,321,236 and 842,022number of transcripts among good and poor quality RNA libraries, respectively. However, a total numberof 3510 and 6759 functional transcripts were identified among good and poor quality bull spermatozoa,respectively. Most of the identified transcripts were related to spermatozoa functions, embryonicdevelopment and other functional aspects of fertilization. Wet laboratory validation of the topfiveselected transcripts (AKAP4, PRM1, ATP2B4, TRIM71 and SLC9B2) illustrated the significant (p
Science and Engineering Research Board
 
Date 2020-04-20T07:56:39Z
2020-04-20T07:56:39Z
2019-01-01
 
Type Research Paper
 
Identifier THERIOGENOLOGY, 129:130-145
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/34907
 
Language English
 
Relation Not Available;
 
Publisher THERIOGENOLOGY