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Prognostic value of post thaw semen quality parameters, mitochondrial integrity and cholesterol content of sperm membrane vis-à-vis conception rate in Frieswal bulls

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Title Prognostic value of post thaw semen quality parameters, mitochondrial integrity and cholesterol content of sperm membrane vis-à-vis conception rate in Frieswal bulls
Not Available
 
Creator SRIVASTAVA N
PANDE M
RAJA T V
TYAGI S
KUMAR S
KUMAR S
KUMAR R
SIROHI A S
CHAND N
ARYA S
KUMAR A
DIN O
 
Subject Cholesterol content, Conception rate, Frieswal bulls, Mitochondrial membrane potential, Quality parameters
 
Description Not Available
The present study aimed to analyze the quantitative relationship of membrane cholesterol content (Chol content),
mitochondrial integrity (∆ψm), and quality parameters of spermatozoa (SQP) at post-thaw stage with conception
rate (CR) and estimated relative conception rate (ERCR) in Frieswal bulls. For the experiment, frozen semen
straws (32) were collected from the SF laboratory and CR (Total insemination 3482) was obtained from Field
Progeny Testing units. Based on the CR, bulls were grouped into low, medium or high groups (G I, G II and G III,
respectively). SQP, viz. viability (Eosin Nigrosin), post thaw motility, biochemical integrity of the membrane
(HOS res), acrosome integrity (Giemsa, and fluorochromes fluorescein isothiocyanate Pisum sativum agglutinin
and propidium iodide, respectively), chol-content, and ∆ψm using fluorescent probe JC-1 (5,5’,6,6’-tetrachloro1,1’,3,3’-tetraethylbenzimi-dazolylcarbocyanine iodide) were determined. The values thus obtained were subjected
to the stepwise regression analysis using the least squares principles for each group, and the CR and ERCR were
regressed on the various SQP. Pearson’s correlation coefficients were estimated between the CR and ERCR values
and the various SQPs. The coefficient of determination (R2) moderately higher for all the models and ranged from
63.70–93.40% (high and medium group, respectively). High R2 value of the prediction equation for the herd and
bulls with medium CR (75.9 and 93.4%, respectively) reveal their suitability to predict the CR and ERCR potential
of the cryopreserved semen. Study results point to inclusion of cholesterol content and ∆ψm estimation in routine
semen analysis before long-term storage or usage for insemination purposes.
Not Available
 
Date 2020-04-20T07:58:21Z
2020-04-20T07:58:21Z
2018-05-18
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/34911
 
Language English
 
Relation Not Available;
 
Publisher INDIAN JOURNAL OF ANIMAL SCIENCES