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Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India

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Title Using PCR-PIRA based genotyping for identifying complex vertebral malformation allele in Frieswal young bulls in India
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Creator ALYETHODI R R
KUMAR S
DEB R
ALEX R
SINGH U
SHARMA S
ASHISH
CHOUDHARY J
SENGAR G S
SINGH R
TYAGI S
PRAKASH B
 
Subject CVM; Frieswal; PCR-PIRA
 
Description Not Available
Complex vertebral malformation (CVM) has considerable economic impact on dairy cattle breeding due to extensive use of artificial insemination (AI). Identifying the carrier is an important factor to reduce the incidence of the genetic disorder. The study was conducted to identify the carriers of CVM in Frieswal cattle by polymerase chain reaction-primer-introduced restriction analysis (PCR-PIRA) method, which was further confirmed by sequencing. Carrier prevalence of 1% was observed in the Frieswal cattle. The results of the study clearly demonstrated the existence of carriers of CVM among Frieswal bull calves. Due to the widespread use of AI it is recommended to screen young bulls at early stages for this defective allele in order to avoid its rapid spread within the population.
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Date 2020-04-20T07:58:44Z
2020-04-20T07:58:44Z
2018-01-01
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/34912
 
Language English
 
Relation Not Available;
 
Publisher Iranian Journal of Veterinary Research, 19(1): 44-47.