Record Details

Identification and molecular cloning of cysteine protease gene of Trypanosoma Evansi Isolated from Camel

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Identification and molecular cloning of cysteine protease gene of Trypanosoma Evansi Isolated from Camel
Not Available
 
Creator Manzer Hakim
Ghorui S.K.
Manohar G.S.
Kashyap S.K.
Kumar N.
Kankar Sashikant
 
Subject Camel
cloning
cysteine protease
Trypanosoma evansi.
 
Description Not Available
A molecular study was carried out to isolate cysteine protease gene of Trypanosoma evansi using PCR. The desired amplicons of cysteine protease gene from the genomic DNA of T. evansi were successfully amplified by PCR using gene specific primers at annealing temperature of 55°C. Amplified PCR product was identified on the basis of its size in agarose gel electrophoresis as 1533 bp. For cloning the purified DNA fragment was ligated to the pGEM-T Easy vector and the ligated mixture was transformed into Escherichia coli JM109 strains. The cells containing recombinant plasmid were identified on the basis of white/blue colony selection on LB agar containing X-Gal, IPTG and ampicillin. Screening of recombinants was done by restriction enzyme digestion of plasmid DNA using EcoRI and confirmed on the basis of gene size, i. e. 1533 bp for cysteine protease gene. Colony PCR was done for quick screening of plasmid inserts directly from E. coli colonies in the presence of insert specific primers.
Not Available
 
Date 2020-04-24T05:43:25Z
2020-04-24T05:43:25Z
2017-01-01
 
Type Article
 
Identifier Not Available
0971-6777
http://krishi.icar.gov.in/jspui/handle/123456789/35049
 
Language English
 
Relation Not Available;
 
Publisher Not Available