Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India
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Title |
Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India
Not Available |
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Creator |
Nagarajan G
Pourouchottamane R Reddy GBM Yogisharadhaya R Sumana K Rajapandi S Murali G Thirumaran SMK Mallick PK Rajendiran AS |
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Subject |
B2L gene
Kodai hills Orf virus phylogenetic analysis sheep |
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Description |
Not Available
Aim: The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu. Materials and Methods: Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools. Results: The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India. Conclusion: The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV. Not Available |
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Date |
2020-05-04T09:35:47Z
2020-05-04T09:35:47Z 2019-07-12 |
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Type |
Research Paper
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Identifier |
Not Available
22310916 http://krishi.icar.gov.in/jspui/handle/123456789/35398 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Veterinary World
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