Record Details

Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Molecular characterization of Orf virus isolates from Kodai hills, Tamil Nadu, India
Not Available
 
Creator Nagarajan G
Pourouchottamane R
Reddy GBM
Yogisharadhaya R
Sumana K
Rajapandi S
Murali G
Thirumaran SMK
Mallick PK
Rajendiran AS
 
Subject B2L gene
Kodai hills
Orf virus
phylogenetic analysis
sheep
 
Description Not Available
Aim:
The present study was carried out to find out the causative agent of exanthematous skin lesions in sheep maintained by Southern Regional Research Centre, Mannavanur, Kodai hills, Tamil Nadu.
Materials and Methods:
Polymerase chain reaction (PCR) with Orf virus (ORFV) B2L gene-specific primers was carried out by employing the total genomic DNA isolated from the scabs as the template. The ORFV isolates from Kodai hills were characterized by the use of bioinformatics tools.
Results:
The amino acid identity of ORFV isolate 1 from Kodai hills is having 98.14%, 96.29%, and 83.59% identity with reference strains of ORFV, Pseudocowpox virus, and bovine papular stomatitis virus, respectively. Phylogenetic analysis revealed that ORFV isolates from Kodai hills clustered with the other ORFV isolates from different geographical areas of India.
Conclusion:
The etiological agent of exanthematous skin lesion among sheep of Kodai hills is ORFV.
Not Available
 
Date 2020-05-04T09:35:47Z
2020-05-04T09:35:47Z
2019-07-12
 
Type Research Paper
 
Identifier Not Available
22310916
http://krishi.icar.gov.in/jspui/handle/123456789/35398
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World