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Differential expression of microRNAs associated with thermal 5 stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle

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Title Differential expression of microRNAs associated with thermal 5 stress in Frieswal (Bos taurus x Bos indicus) crossbred dairy cattle
Not Available
 
Creator Gyanendra Singh Sengar
Rajib Deb
Umesh Singh
T. V. Raja
Rajiv Kant
Basavraj Sajjanar
Rani Alex
R. R. Alyethodi
Ashish Kumar
Sushil Kumar
Rani Singh
Subhash J
Jakhesara
C. G. Joshi
 
Subject Heat stress .miRNAs . Frieswal . bta-miR-2898 . 41 HSPB8
 
Description Not Available
Environmental temperature is one of the important abiotic factors
that influence the normal physiological function and productive
performance of dairy cattle. Temperature stress evokes complex
responses that are essential for safeguarding of cellular integrity
and animal health. Post-transcriptional regulation of gene
expression by miRNA plays a key role cellular stress responses. The
present study investigated the differential expression of miRNA in
Frieswal (Holstein Friesian × Sahiwal) crossbred dairy cattle that are
distinctly adapted to environmental temperature stress as they were
evolved by using the temperate dairy breed Holstein Friesian. The
results indicated that there was a significant variation in the
physiological and biochemical indicators estimated under summer
stress. The differential expression of miRNA was observed under
heat stress when compared to the normal winter season. Out of the
total 420 miRNAs, 65 were differentially expressed during peak
summer temperatures. Most of these miRNAs were found to target
heat shock responsive genes especially members of heat shock
protein (HSP) family, and network analysis revealed most of them
having stress-mediated effects on signaling mechanisms. Being
greater in their expression profile during peak summer,
bta-miR-2898 was chosen for reporter assay to identify its effect on
the target HSPB8 (heat shock protein 22) gene in stressed bovine
PBMC cell cultured model. Comprehensive understanding of the
biological regulation of stress responsive mechanism is critical for
developing approaches to reduce the production losses due to
environmental heat stress in dairy cattle.
Science and Engineering Research 712 Board, Government of India
 
Date 2020-05-20T06:15:54Z
2020-05-20T06:15:54Z
2017-07-19
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/35854
 
Language English
 
Relation Not Available;
 
Publisher Not Available