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Effect of heat stress on the expression profile of Hsp90 among Sahiwal (Bos indicus) and Frieswal (Bos indicus × Bos taurus) breed of cattle: A comparative study

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Title Effect of heat stress on the expression profile of Hsp90 among Sahiwal (Bos indicus) and Frieswal (Bos indicus × Bos taurus) breed of cattle: A comparative study
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Creator Rajib Deb ⁎,1, Basavaraj Sajjanar 1,2, Umesh Singh, Sushil Kumar, Rani Singh, G. Sengar, Arjava Sharma
 
Subject Heat shock protein 90 Expression Heat stress Frieswal Sahiwal
 
Description Not Available
We evaluated the effect of thermal challenge on the expression profile of heat shock protein 90 (Hsp90) among
Sahiwal (Bos indicus) and Frieswal (Bos indicus × Bos taurus) breeds of cattle. The present investigation was focused
on the comparative studies on Hsp90 expression among Frieswal and Sahiwal under in vitro and environmental
heat stress. Measured immediately after the in vitro heat shock to the peripheral blood mononuclear cells
(PBMCs), the relative expression of Hsp90 mRNA was significantly (P b 0.05) higher in Sahiwal compared to
those in Frieswal. In later intervals of time, the differences in the expression levels between the two breeds
become negligible coming down towards the basal level. A similar pattern was observed in the protein concentration
showing significantly (P b 0.05) higher levels in Sahiwal compared to those in Frieswal. The second sets of
experiments were undertaken during summer months (March to May) when temperature peaked from 37 to
45 °C. During these months, Frieswal cows consistently recorded higher rectal temperatures than the Sahiwal
breed. Further during this peak summer stress, Sahiwal showed significantly higher levels of mRNA transcripts
as well as protein concentration compared to the Frieswal breed. Our findings also interestingly showed that,
the cell viability of PBMC are significantly higher among the Sahiwal than Frieswal. Taken together, the experiments
of both induced in vitro and environmental stress conditions indicate that, Sahiwal may express higher
levels of Hsp90 then Frieswal to regulate their body temperature and increase cell survivality under heat stressed
conditions.
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Date 2020-05-20T06:16:25Z
2020-05-20T06:16:25Z
2013-12-11
 
Type Research Paper
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/35855
 
Language English
 
Relation Not Available;
 
Publisher ELSEVIER