Development of a tetra-primer ARMS PCR-based assay for detection of a novel single-nucleotide polymorphism in the 50 untranslated region of the bovine ITGB6 receptor gene associated with foot-and-mouth disease susceptibility in cattle
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Title |
Development of a tetra-primer ARMS PCR-based assay for detection of a novel single-nucleotide polymorphism in the 50 untranslated region of the bovine ITGB6 receptor gene associated with foot-and-mouth disease susceptibility in cattle
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Creator |
Rani Singh • Rajib Deb • Umesh Singh • Rani Alex • Sushil Kumar • Soumendu Chakraborti • Sheetal Sharma • Gyanendra Sengar • Rupali Singh
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Subject |
ARMS PCR ITGB6 Cattle FMDV 50UTR
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Description |
Not Available
ITGB6 is known to be one of the major receptor components involved in host tropism of foot-andmouth disease (FMD) virus in cattle. A competitive PCR technique called ARMS PCR was adapted to identify a single-nucleotide polymorphism (SNP), G29A, db SNP Id: rs109075046, in the 50 untranslated region (50UTR) of the bovine ITGB6 gene. Genotype profiling identified three kinds of genetic variation within the targeted SNP among Frieswal crossbred cattle. The occurrence of FMD in the three genotypes was further evaluated, revealing a clear role in the incidence of FMD in the studied population |
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Date |
2020-05-20T06:16:39Z
2020-05-20T06:16:39Z 2014-07-21 |
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Type |
Research Paper
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Identifier |
Not Available
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/35856 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Springer
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