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Functional proteomic analysis of crossbred (Holstein Friesian × Sahiwal) bull spermatozoa

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Title Functional proteomic analysis of crossbred (Holstein Friesian × Sahiwal) bull spermatozoa
Not Available
 
Creator R Singh,GS Sengar, U Singh ,R Deb, V Junghare,  S Hazra, S Kumar,  S Tyagi, AK Das ,TV Raja, A Kumar
 
Subject frozen semen ,spermatozoal concentration, initial progressive motility, TUNEL
 
Description Not Available
Male infertility is one of the prime concerns of dairy cattle production. The study was
designed to find out differentially expressed proteins in categorized crossbred
(Holstein Friesian × Sahiwal) bull semen to serve as potential biomarkers for male in-
fertility. Frozen crossbred bull semen with satisfactory phenotypic records were de-
fined as “good” and “poor” based on their fertility rates. A total of 1,547 proteins were
detected in bull spermatozoa using liquid chromatography–mass spectrometer (LC-
MS/MS) analysis. Results revealed that 558 (36.1%) and 653 (42.2%) proteins were
expressed to good and poor quality bull spermatozoa, respectively. A total of 336
proteins (21.7%) were reported to be unique for both good and poor quality bull
semen, and among the common proteins, 224 (66.7%) and 112 (33.3%) were up- and
downregulated in good and poor quality categorized bull semen, respectively. Gene
Ontology analysis of global proteomes identified different signalling pathways, and
most of them were related to cellular motility, immune systems as well as cellular me-
tabolisms. The distinctive presence of some of the proteins may provide an insight into
the molecular mechanistic role played by these proteins in crossbred bull infertility
SERB ,GOI
 
Date 2020-05-20T06:17:01Z
2020-05-20T06:17:01Z
2018-01-10
 
Type Research Paper
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/35857
 
Language English
 
Relation Not Available;
 
Publisher Wiley