Effect of filtration through sephadex and glasswool on the quality and freezability of semen of crossbred bulls
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Title |
Effect of filtration through sephadex and glasswool on the quality and freezability of semen of crossbred bulls
Not Available |
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Creator |
Sumant Vyas
Greesh Mohan A.J. Dhami K.L. Sahni |
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Subject |
sephadex
glasswool filtration Semen quality and freezability crossbred bulls |
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Description |
Not Available
Nearly 30 % of crossbred bulls donalte poor quality semen with low freezability rendering them unfit for AI (Pathak 1988, Sahni and Mohan 1988). The objective of the present study was to determine whether glass-wool and sephadex- gel column filtration could improve the quality and freezability of semen of triple crossbred bulls. Ejaculates (40) from 5 triple crossbred bulls (1/2 HFx1/4Jx1/4H) were collected weekly with an artificial vagina and used in a split sample technique. The bulls were maintained under identical conditions at the germplasm centre of the institute. Slurries of sephadex G-25 (12 %w/v) and G-50 (6% w/v) (Pharmacia Co. Uppsala, Sweden) and glasswool filter were prepared and semen was filtered according to Vyas et al. (1991). The samples were then diluted with tris-citric acid-fructose-yolk glycerol diluent to give 20-25 millions sperm/0.54 ml French medium straws and frozen in liquid nitrogen after 3 hr equilibration at 5 0 C. The straws were thawed at 38 0 C. The percentage of motile sperm in initial, pre-freeze and post thaw (0 hr and 1 he incubation) samples was assessed with a phase-contrast microscope (40x) fitted with a biotherm stage. Similarly we determined at initial and post-thaw live/dead and abnormal (head, mid-piece, tail and total) spermatozoa. The data were analysed statistically according to Snedecor and Cochran (1967). Not Available |
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Date |
2020-07-03T10:21:00Z
2020-07-03T10:21:00Z 1992-04-01 |
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Type |
Article
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Identifier |
Not Available
0367-8318 http://krishi.icar.gov.in/jspui/handle/123456789/38011 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Indian Council of Agricultural Research
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