Record Details

Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken
Not Available
 
Creator Pranay Kumar
B. Swathi
Shanmugam M
 
Subject Butylated hydroxy toluene; chicken; cryopreservation; fertility; semen; trolox
 
Description Not Available
The effect of supplementing water-soluble vitamin E analogues 6-hydroxy-2,5,7,8-tetramethylchromane-
2-carboxylic acid (trolox) and butylated hydroxy toluene (BHT) was studied in two
separate experiments.
2. In the first experiment, trolox was supplemented at 0.2mM, 0.4mMand 0.8mMconcentrations along
with N-methylacetamide (MA; 12% final concentration) and semen was cryopreserved in 0.5 ml French
straws. Different semen parameters and fertility were assessed from post-thaw samples.
3. Sperm motility, live sperm, and mitochondrial activity were significantly lower (P < 0.05) in
cryopreserved semen. Lipid peroxidation (LPO) was significantly higher (P < 0.05) in cryopreserved
semen that was reduced by trolox supplementation. The treatment containing trolox at 0.2 mM
concentration produced significantly higher (P < 0.05) fertility compared to unsupplemented
cryopreservation treatment.
4. In the second experiment, BHT was supplemented at 0.25 mM, 0.5 mM, and 1 mM concentrations
along with MA during semen cryopreservation.
5. Sperm motility, live sperm and MTT dye reduction test were significantly lower (P < 0.05) in
cryopreserved semen. These parameters declined with increasing BHT concentration. Abnormal
sperm was significantly higher (P < 0.05) in the BHT supplemented treatments. The sperm chromatin
dispersion (SCD) test was significantly higher (P < 0.05) in cryopreserved samples and was highest in
samples supplemented with 0.5 mM and 1 mM BHT. The percentage fertility was significantly lower
(P < 0.05) in cryopreserved semen and BHT supplementation did not improve fertility.
6. In conclusion, trolox supplementation at 0.2 mM concentration during semen cryopreservation
improved fertility, whereas BHT supplementation resulted in a decline in post-thaw semen
parameters.
Not Available
 
Date 2020-06-26T11:25:24Z
2020-06-26T11:25:24Z
2019-03-01
 
Type Research Paper
 
Identifier K. Pranay Kumar, B. Swathi & M. Shanmugam (2019) Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken, British Poultry Science, 60:3, 340-345, DOI: 10.1080/00071668.2019.1602249
0007-1668
http://krishi.icar.gov.in/jspui/handle/123456789/37450
 
Language English
 
Relation Not Available;
 
Publisher Taylor & Francis