Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken
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Title |
Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken
Not Available |
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Creator |
Pranay Kumar
B. Swathi Shanmugam M |
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Subject |
Butylated hydroxy toluene; chicken; cryopreservation; fertility; semen; trolox
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Description |
Not Available
The effect of supplementing water-soluble vitamin E analogues 6-hydroxy-2,5,7,8-tetramethylchromane- 2-carboxylic acid (trolox) and butylated hydroxy toluene (BHT) was studied in two separate experiments. 2. In the first experiment, trolox was supplemented at 0.2mM, 0.4mMand 0.8mMconcentrations along with N-methylacetamide (MA; 12% final concentration) and semen was cryopreserved in 0.5 ml French straws. Different semen parameters and fertility were assessed from post-thaw samples. 3. Sperm motility, live sperm, and mitochondrial activity were significantly lower (P < 0.05) in cryopreserved semen. Lipid peroxidation (LPO) was significantly higher (P < 0.05) in cryopreserved semen that was reduced by trolox supplementation. The treatment containing trolox at 0.2 mM concentration produced significantly higher (P < 0.05) fertility compared to unsupplemented cryopreservation treatment. 4. In the second experiment, BHT was supplemented at 0.25 mM, 0.5 mM, and 1 mM concentrations along with MA during semen cryopreservation. 5. Sperm motility, live sperm and MTT dye reduction test were significantly lower (P < 0.05) in cryopreserved semen. These parameters declined with increasing BHT concentration. Abnormal sperm was significantly higher (P < 0.05) in the BHT supplemented treatments. The sperm chromatin dispersion (SCD) test was significantly higher (P < 0.05) in cryopreserved samples and was highest in samples supplemented with 0.5 mM and 1 mM BHT. The percentage fertility was significantly lower (P < 0.05) in cryopreserved semen and BHT supplementation did not improve fertility. 6. In conclusion, trolox supplementation at 0.2 mM concentration during semen cryopreservation improved fertility, whereas BHT supplementation resulted in a decline in post-thaw semen parameters. Not Available |
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Date |
2020-06-26T11:25:24Z
2020-06-26T11:25:24Z 2019-03-01 |
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Type |
Research Paper
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Identifier |
K. Pranay Kumar, B. Swathi & M. Shanmugam (2019) Effect of supplementing vitamin E analogues on post-thaw semen parameters and fertility in chicken, British Poultry Science, 60:3, 340-345, DOI: 10.1080/00071668.2019.1602249
0007-1668 http://krishi.icar.gov.in/jspui/handle/123456789/37450 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Taylor & Francis
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