Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature
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Title |
Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature
Not Available |
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Creator |
Shanmugam M
R.K.Mahapatra |
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Subject |
chicken, cryoprotectant, diluent, fertility, semen cryopreservation
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Description |
Not Available
The present study evaluated the effects of cryoprotectants, semen diluents and thawing temperature during Ghagus chicken semen cryopreservation. Four different experiments were conducted; Experiment 1—semen was cryopreserved using 6% dimethylacetamide (DMA) and 2% dimethylsulphoxide (DMSO) in Sasaki diluent (SD) and Lake and Ravie diluent (LR), Experiment 2 and 3—semen was cryopreserved using 8% ethylene glycol (EG) in SD, LRD and Red Fowl Extender (RFE), Experiment 4—semen was cryopreserved using 6% dimethylformamide (DMF) in SD, LR and Beltsville poultry semen extender (BPSE). Semen was cryopreserved in 0.5 ml French straws. Thawing was done at 5°C for 100 s in ice water in Experiments 1, 2 and 4, whereas in Experiment 3 thawing was done at 37°C for 30 s. The post-thaw sperm motility, viable sperm and acrosome-intact sperm were significantly (p < .05) lower in cryopreserved samples in all the experiments. No fertile eggs were obtained from cryopreserved samples in Experiments 1 and 2, except for 8% EG RFE treatment where the fertility was 0.83%. In Experiments 3 and 4, highest fertility was obtained in LR treatment 48.12 and 30.89%, respectively. In conclusion, using cryoprotectant EG (8%) and thawing at 37°C for 30 s, and DMF(6%) resulted in acceptable level of fertility in Ghagus chicken. Though the diluents influenced post-thaw in vitro semen parameters, the fertility was not affected. In addition, results indicated that thawing temperature may be a critical stage in the cryopreservation protocol. Not Available |
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Date |
2020-06-26T11:26:35Z
2020-06-26T11:26:35Z 2020-06-01 |
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Type |
Research Paper
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Identifier |
Murugesan S, Mahapatra R. Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature. Reprod Dom Anim. 2020;00:1–7. https://doi.org/10.1111/rda.13734
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/37451 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Wiley
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