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Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature

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Title Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature
Not Available
 
Creator Shanmugam M
R.K.Mahapatra
 
Subject chicken, cryoprotectant, diluent, fertility, semen cryopreservation
 
Description Not Available
The present study evaluated the effects of cryoprotectants, semen diluents and
thawing temperature during Ghagus chicken semen cryopreservation. Four different
experiments were conducted; Experiment 1—semen was cryopreserved using
6% dimethylacetamide (DMA) and 2% dimethylsulphoxide (DMSO) in Sasaki diluent
(SD) and Lake and Ravie diluent (LR), Experiment 2 and 3—semen was cryopreserved
using 8% ethylene glycol (EG) in SD, LRD and Red Fowl Extender (RFE), Experiment
4—semen was cryopreserved using 6% dimethylformamide (DMF) in SD, LR and
Beltsville poultry semen extender (BPSE). Semen was cryopreserved in 0.5 ml French
straws. Thawing was done at 5°C for 100 s in ice water in Experiments 1, 2 and 4,
whereas in Experiment 3 thawing was done at 37°C for 30 s. The post-thaw sperm
motility, viable sperm and acrosome-intact sperm were significantly (p < .05) lower
in cryopreserved samples in all the experiments. No fertile eggs were obtained from
cryopreserved samples in Experiments 1 and 2, except for 8% EG RFE treatment
where the fertility was 0.83%. In Experiments 3 and 4, highest fertility was obtained
in LR treatment 48.12 and 30.89%, respectively. In conclusion, using cryoprotectant
EG (8%) and thawing at 37°C for 30 s, and DMF(6%) resulted in acceptable level of
fertility in Ghagus chicken. Though the diluents influenced post-thaw in vitro semen
parameters, the fertility was not affected. In addition, results indicated that thawing
temperature may be a critical stage in the cryopreservation protocol.
Not Available
 
Date 2020-06-26T11:26:35Z
2020-06-26T11:26:35Z
2020-06-01
 
Type Research Paper
 
Identifier Murugesan S, Mahapatra R. Cryopreservation of Ghagus chicken semen: Effect of cryoprotectants, diluents and thawing temperature. Reprod Dom Anim. 2020;00:1–7. https://doi.org/10.1111/rda.13734
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/37451
 
Language English
 
Relation Not Available;
 
Publisher Wiley