Development and evaluation of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Assay for differentiation of foot and mouth disease virus serotype O lineages circulating in India
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Title |
Development and evaluation of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Assay for differentiation of foot and mouth disease virus serotype O lineages circulating in India
Not Available |
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Creator |
Subramaniam S, Rekha G, Biswal JK, Ranjan R, Pattnaik B
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Subject |
287-291
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Description |
Not Available
Foot and mouth disease (FMD) is endemic in India and among the three prevalent serotypes (O, A and Asial), serotype O causes the majority of the FMD incidences. Phylogenetic analysis based on the VP1 coding region sequences of FMD serotype O viruses from India has revealed the circulation of two major genetic lineages (Ind2001 and Pan Asia) in recent years. Timely determination of lineage in circulation is essential for the effective implementation of FMD control. In present study, a rapid and simple lineage PCR assay to differentiate present genetic lineages of serotype O viruses was developed and evaluated. Stretches of lineage-specific signature residues were identified and primers were designed. The lineage differentiating PCR assay can be very useful for preliminary identification of serotype O lineages circulating in the country before proceeding with the sequencing of the VP1 coding region for confirmation, which is time-consuming. Not Available |
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Date |
2021-02-09T05:39:14Z
2021-02-09T05:39:14Z 2018-12-01 |
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Type |
Research Paper
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Identifier |
Subramaniam S, Rekha G, Biswal JK, Ranjan R, Pattnaik B (2019). Development and evaluation of Reverse Transcription-Polymerase Chain Reaction (RT-PCR) Assay for differentiation of foot and mouth disease virus serotype O lineages circulating in India. Indian J. Vet. Pathol. 42(4), 287-291
Not Available http://krishi.icar.gov.in/jspui/handle/123456789/45292 |
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Language |
English
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Relation |
Not Available;
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Publisher |
Diva enterprises Pvt Ltd
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