Development and validation of high throughput real-time polymerase chain reaction assay for quantitative detection of chicken infectious anemia virus
KRISHI: Publication and Data Inventory Repository
View Archive InfoField | Value | |
Title |
Development and validation of high throughput real-time polymerase chain reaction assay for quantitative detection of chicken infectious anemia virus
Not Available |
|
Creator |
T. R. Kannaki
E. Priyanka Madhuri Subbiah Santosh Haunshi |
|
Subject |
Chicken infectious anemia ∙ Real-time PCR
|
|
Description |
Not Available
A real time polymerase chain reaction (real-time PCR) assay was developed to detect and quantify the chicken infectious anemia virus (CIAV). The two sets of primers specific to VP1 region of CIAV were designed and their sensitivity and efficacy were studied. Both the primers designed in this study were highly sensitive and were able to detect upto 0.01 fg/µl or 82 x 102 copy number of plasmid DNA. The efficiency of the real time PCR was 100.9 %. The results have also shown that the present qPCR assay is 100 times more sensitive than regular qualitative PCR. Both primer sets were validated using 28 field poultry samples and showed good results. The optimized real-time quantitative PCR will be useful in quick detection of field outbreaks, sub-clinical infection in poultry flocks, virus pathogenesis studies and for detecting vaccine contamination. Not Available |
|
Date |
2021-04-12T09:33:45Z
2021-04-12T09:33:45Z 2021-04-01 |
|
Type |
Research Paper
|
|
Identifier |
10.1007/s13337-020-00648-1
http://krishi.icar.gov.in/jspui/handle/123456789/46429 |
|
Language |
English
|
|
Relation |
Not Available;
|
|
Publisher |
Springer
|
|