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Development of recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay for sero-survey of porcine reproductive and respiratory syndrome

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Title Development of recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay for sero-survey of porcine reproductive and respiratory syndrome
Not Available
 
Creator Kashyap SP
Hiremath J
Vinutha S
Patil SS
Suresh KP
Roy P
Hemadri D
 
Subject indirect enzyme-linked immunosorbent assay
nucleocapsid protein
porcine reproductive and respiratory syndrome
sensitivity
specificity
 
Description Not Available
Background and Aim: Porcine reproductive and respiratory syndrome (PRRS) is a disease endemic in many countries and is of economic importance. India was free from PRRS until the first outbreak was reported from a North-East Indian state in 2013. Since then, disease outbreaks have been reported from North-East India and the pilot study conducted earlier showed that it is gradually spreading to the rest of India. Considering there are no locally developed population screening tests available for PRRS and imported diagnostic/screening tests are expensive, the present study was aimed at developing recombinant nucleocapsid (rN) protein-based indirect enzyme-linked immunosorbent assay (iELISA). Materials and Methods: The rN protein of PRRS virus (PRRSV) was produced following standard cloning, expression, and purification procedures. Using this antigen, iELISA was optimized for the detection of serum antibodies to PRRSV. The sensitivity and specificity of the test were assessed by comparing it with a commercial PRRSV antibody detection kit. Results: A total of 745 serum samples from ten different states of India were screened using the developed iELISA. The iELISA had a relative specificity of 76.18% and sensitivity of 82.61% compared to the commercial ELISA (Priocheck PRRSV ELISA kit, Thermo Fisher Scientific, USA). Conclusion: The iELISA, which deployed rN protein from Indian PRRSV, was found to be suitable in the serological survey and may be a useful tool in future disease surveillance programs.
Not Available
 
Date 2021-04-22T03:59:48Z
2021-04-22T03:59:48Z
2020-12-05
 
Type Research Paper
 
Identifier Kashyap SP, Hiremath J, Vinutha S, Patil SS, Suresh KP, Roy P and Hemadri D. (2020). Development of recombinant nucleocapsid protein-based indirect enzyme-linked immunosorbent assay for sero-survey of porcine reproductive and respiratory syndrome. Veterinary World. 13(12): 2587-2595.
0972-8988 (Print)
2231-0916 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/46568
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World