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In silico study of Ceftazidime and Piperacillin against Penicillin-Binding Protein 2, Beta-Lactamase (OXA-1 and SHV-28) of Klebsiella pneumoniae U25

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Title In silico study of Ceftazidime and Piperacillin against Penicillin-Binding Protein 2, Beta-Lactamase (OXA-1 and SHV-28) of Klebsiella pneumoniae U25
Not Available
 
Creator Kendaganna PH
Shruthi G
Karunakar P
Patil S
Shiva Prasad K
Chandrashekar S
Chandan S
 
Subject Beta-lactamases
Ceftazidime
Docking interactions
Klebsiella pneumoniae U25
Penicillin-Binding Protein 2
Piperacillin
 
Description Not Available
To combat the action of beta-lactams, Klebsiella pneumoniae launches the enzymatic action by producing beta-lactamases to destruct the activity of ceftazidime and piperacillin. Objective: In our research, we want to know the action of ceftazidime and piperacillin on penicillin-binding protein2 (PBP2) and also does it have any interactions with beta-lactamases (OXA1 and SHV-28). Our idea is to prevent the action of beta-lactamases on
ceftazidime and piperacillin. Hence, we have modified the beta-lactam core structures of ceftazidime and piperacillin and done the comparative docking interaction studies. Materials and Methods: K. pneumoniae U25 has been selected for the comparative docking analysis study with ceftazidime and piperacillin antibiotics by modifying its structures and targeting them against PBP2 and beta-lactamases (OXA-1 and SHV-28). Results: Our docking analysis revealed that ceftazidime and modified ceftazidime are forming hydrogen bonds, but piperacillin and modified piperacillin are showing hydrophobic interactions with an active site serine residue (Ser316) of PBP2 responsible for the transpeptidase activity in K. pneumoniae U25. Protective action by beta-lactamases (OXA-1 and SHV-28) to K. pneumoniae U25 against beta-lactam antibiotics is also revealed through our study by docking interactions of Ser71 of OXA-1 and Ser66 of SHV-28 with the ceftazidime, modified ceftazidime, piperacillin, and modified piperacillin.
Not Available
 
Date 2021-04-22T04:01:35Z
2021-04-22T04:01:35Z
2020-11-11
 
Type Research Paper
 
Identifier Kendaganna PH, Shruthi G, Karunakar P, Patil S, Shiva Prasad K, Chandrashekar S and Chandan S. (2020). In silico study of Ceftazidime and Piperacillin against Penicillin-Binding Protein 2, Beta-Lactamase (OXA-1 and SHV-28) of Klebsiella pneumoniae U25. International Journal of Green Pharmacy. 14(4): 1-9.
1998-4103 (Online)
0973-8258 (Print)
http://krishi.icar.gov.in/jspui/handle/123456789/46569
 
Language English
 
Relation Not Available;
 
Publisher Medknow Publications