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Comparative genetic diversity analysis based on virulence and repetitive gene profiling of Pasteurella multocida isolates from animal hosts

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Title Comparative genetic diversity analysis based on virulence and repetitive gene profiling of Pasteurella multocida isolates from animal hosts
Not Available
 
Creator Prajapati A
Chanda MM
Yogisharadhya R
Parveen A
Ummer J
Dhayalan A
Mohanty NN
Shivachandra SB
 
Subject Pasteurella multocida
PCR
Repetitive elements
Virulence associated genes
 
Description Not Available
Virulence associated and/or housekeeping/repetitive genes either in single or multiple copies are being extensively targeted for bacterial pathogen detection and differentiation in epidemiological studies. In the present study, isolation of Pasteurella multocida from different animals and their genetic profiling based on the capsular types, virulence and repetitive elements (ERIC/REP) were carried out. A total 345 clinical samples from apparently healthy and diseased (pneumonic, septicaemia) animals (sheep, goat, pig, cattle, buffalo and rabbits) from different geographical regions of Karnataka, Uttar Pradesh, Mizoram and Assam states of India were screened. A total of 32% of the samples were found positive, of which 41 P. multocida isolates recovered.
Virulence profiling of isolates indicated that omp87, ompA, ptfA, sodA, sodC, nanB, fur and exbB were present in 100% of isolates. Whereas, prevalence of other genes were; nanH (90%), ompH (71%), pfhA (63%), plpB (80%), hsf-1 (12%), hsf-2 (37%), pmHAS (78%), toxA (73%), hgbA (37%), hgbB (81%), tbpA (78%) and fimA (98%), among isolates. There was no influence of host or place on prevalence of virulence genes when assessed by fitting a Hierarchial Bayesian ordinal regression model. There was correlation (positive and negative) between broad groups of virulence genes. Both repetitive gene profiles (ERIC and REP) generated multiple amplicons (~200 to ~4000 bp). Cluster analysis with ERIC profiles revealed 5 clusters and 3 non- typable isolates with higher discriminatory power (D = 0.7991) than the REP-PCR profiles (D = 00.734) which revealed 4 clusters and 6 non- typable isolates. The results showed that a considerable level of genetic diversity exists among circulating P. multocida isolates despite belonging to the same geographical origin. The genetic diversity or clustering based on either virulence or repetitive elements among isolates could be largely driven by multiple factors acting together which lead to manifestations of particular disease symptoms.
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Date 2021-04-22T04:18:31Z
2021-04-22T04:18:31Z
2020-09-17
 
Type Research Paper
 
Identifier Prajapati A, Chanda MM, Yogisharadhya R, Parveen A, Ummer J, Dhayalan A, Mohanty NN and Shivachandra SB. (2020). Comparative genetic diversity analysis based on virulence and repetitive gene profiling of Pasteurella multocida isolates from animal hosts. Infection, Genetics and Evolution. 85: 104564. 1-11.
1567-1348 (Print)
1567-7257 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/46582
 
Language English
 
Relation Not Available;
 
Publisher Elsevier Publishers private limited