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Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid-gestation

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Title Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid-gestation
Not Available
 
Creator Saminathan M
Singh KP
Vineetha S
Maity M
Biswas SK
Reddy GBM
Chauhan HC
Milton AAP
Ramakrishnan MA
Maan S
Maan NS
Hemadri D
Chandel BS
Gupta VK
Mertens PPC
 
Subject Transplacental transmission
bluetongue virus
BTV-1
 
Description Not Available
Transplacental transmission (TPT) of wild-type Indian BTV-1 had never been experimentally proved. This study was first time investigated TPT of Indian BTV-1 (isolated from aborted and stillborn goat fetal spleens). The sequential pathology, virological and immune cell kinetics (CD4+, CD8+ T-lymphocytes and NK cells in spleen and PBMCs), and apoptosis in IFNAR1-blocked pregnant mice during early (infected on 1 GD) and mid (infected on 8 GD) gestation have been studied. There was higher rate of TPT during mid stage (71.43%) than early (57.14%) stage. In early stage reduced implantation sites, early embryonic deaths, abortions, and necro-haemorrhagic lesions had observed. Mid stage, congenital defects and neurological lesions in foetuses like haemorrhages, diffuse cerebral edema, necrotizing encephalitis and decreased bone size (Alizarin red staining) were noticed. BTV-1 antigen was first time demonstrable in cells of mesometrium, decidua of embryos, placenta, uterus, ovary, and brain of foetuses by immunohistochemistry and quantified by real-time qRT-PCR. BTV-inoculated mice were seroconverted by 7 and 5 dpi, and reached peak levels by 15 and 9 dpi in early and mid gestation, respectively. CD4+ and CD8+ cells were significantly decreased (increased ratio) on 7 dpi but subsequently increased on 15 dpi in early gestation. In mid gestation, increased CD8+ cells (decreased ratio) were observed. Apoptotic cells in PBMCs and tissues increased during peak viral load. This first time TPT of wild-type Indian BTV-1 deserves to be reported for implementation of control strategies. This model will be very suitable for further research into mechanisms of TPT, overwintering, and vaccination strategies.
Not Available
 
Date 2021-04-22T04:25:11Z
2021-04-22T04:25:11Z
2020-02-07
 
Type Research Paper
 
Identifier Saminathan M, Singh KP, Vineetha S, Maity M, Biswas SK, Reddy GBM, Chauhan HC, Milton AAP, Ramakrishnan MA, Maan S, Maan NS, Hemadri D, Chandel BS, Gupta VK and Mertens PPC. 2020. Virological, immunological and pathological findings of transplacentally transmitted bluetongue virus serotype 1 in IFNAR1-blocked mice during early and mid-gestation. Scientific Reports. 10: 2164. https://doi.org/10.1038/s41598-020-58268-0
2045-2322
http://krishi.icar.gov.in/jspui/handle/123456789/46589
 
Language English
 
Relation Not Available;
 
Publisher Nature Research