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Molecular characterization of sheep pox virus from outbreaks in Karnataka, India

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Title Molecular characterization of sheep pox virus from outbreaks in Karnataka, India
Not Available
 
Creator Sumana K
Yogisharadhya R
Apsana R
Roy P
Reddy GBM
 
Subject goatpox
P32
polymerase chain reaction
phylogenetic analysis
sheeppox
 
Description Not Available
Aim: This study aimed to characterize sheeppox virus (SPPV) using the P32 gene of the Capripoxvirus (CaPVs). Materials and Methods: Clinical samples of skin, scabs, and nasal swab from suspected outbreaks Horalagallu (n=13) and Gerahalli (n=11) at Ramanagara district in Karnataka were collected. All the samples were initially subjected to genusspecific diagnostic polymerase chain reaction (PCR). The pooled clinical samples from each outbreak were also subjected to virus isolation. The isolates were confirmed by CaPVs genotyping PCR targeting the full-length P32 gene, followed by sequencing and phylogenetic analysis.
Results: The clinical signs and lesions varied from mild to severe degree with no specificity between age and sex. Specific cytopathic changes in cell morphology were observed in infected Vero cells from both outbreaks, which were confirmed by PCR. The complete P32 gene from two outbreaks was successfully amplified with the expected amplicon size of 1006bp. The sequencing and phylogenetic analysis revealed that both the outbreaks were due to SPPV and shared high similarity with published SPPVs from Karnataka and other parts of India.
Conclusion: The current study showed that complete P32 gene-based genotypic PCR assay can be used for genetic characterization and molecular epidemiology of both sheeppox and goatpox diseases and also to differentiate the causative agents. The sequence analysis revealed 100% similarity among the two outbreak isolates suggesting the same strain of the virus and common source of infection for the outbreaks.
Not Available
 
Date 2021-04-22T04:39:25Z
2021-04-22T04:39:25Z
2020-02-28
 
Type Research Paper
 
Identifier Sumana K, Yogisharadhya R, Apsana R, Roy P and Reddy GBM. (2020). Molecular characterization of sheep pox virus from outbreaks in Karnataka, India. Veterinary World. 13(2): 386-391. DOI: 10.14202/vetworld.2020.386-391.
0972-8988 (Print)
2231-0916 (Online)
http://krishi.icar.gov.in/jspui/handle/123456789/46598
 
Language English
 
Relation Not Available;
 
Publisher Veterinary World