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Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

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Title Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep
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Creator Ganesh G Sonawane, Bhupendra Nath Tripathi, Rajiv Kumar, Jyoti Kumar
 
Subject diagnosis, lung tissues, ovine pulmonary adenomatosis, sheep
 
Description Not Available
Aim: This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary
disorders at organized farm.
Materials and Methods: A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during
necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into
two parts; one each for polymerase chain reaction (PCR) and another for histopathology.
Results: On PCR examination of lung tissues, six sheep (8%) were found to be positive for JSRV. Two of them were
3-6 months of age and did not show clinical signs/gross lesions of OPA. Four adult sheep positive on PCR revealed
characteristic lesions of OPA on gross and histopathological examination.
Conclusion: In the absence of known specific antibody response to the infection with JSRV, there is no diagnostic serological
test available. The PCR assay employed in this study on lung tissues, using primers based on the U3 region of the viral long
terminal repeat for JSRV would be helpful in the screening of preclinical and clinical cases of OPA in sheep.
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Date 2021-07-15T09:14:42Z
2021-07-15T09:14:42Z
2016-04-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/48127
 
Language English
 
Relation Not Available;
 
Publisher Not Available