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DETECTION OF IN CULTURE AND LUNG TISSUE OF LAMBS MANNHEIMIA HAEMOLYTICA BY REAL-TIME POLYMERASE CHAIN REACTION ASSAY

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Title DETECTION OF IN CULTURE AND LUNG TISSUE OF LAMBS MANNHEIMIA HAEMOLYTICA BY REAL-TIME POLYMERASE CHAIN REACTION ASSAY
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Creator Jyoti Kumar, GG Sonawane, SJ Pandian, Rajiv Kumar1 CP Swarnkar
 
Subject Lamb, Lung,Mannheimia haemolytica,Pneumonia,Real-time polymerase chain reaction, Sensitivity
 
Description Not Available
Mannheimia haemolytica is one of the bacterial species involved in cases of ovine respiratory
complex, causing significant economic loss to sheep production worldwide. In the year 2016-17, a
study was undertaken with the aim to evaluate a SYBR Green dye based real-time polymerase
chain reaction (PCR) assay targeting O-sialo glycoprotein endopeptidase (gcp) gene for the
detection of M. haemolytica in the DNA isolated from culture and pneumonic lung tissue of
neonatal lambs. The test showed thousand times more sensitivity than conventional PCR and
detected down to 100 fg of genomic DNA of pure M. haemolytica. The real-time PCR was found
specific for gcp gene ofM. haemolytica, as no cross reactivitywas detectedwith a variety of known
bacterial isolates characterized previously by species specific or 16S rRNA PCR. The real-time
PCR was employed to screen M. haemolytica in 41 ovine lung tissues collected from neonatal
lambs, which showed increased level of detection as compared to the conventional PCR. The
specificity of the PCR products was confirmed by sequencing of the amplified products of 227 bp
size that showed 99-100% homology with the published sequences available in the NCBI
database. It was concluded that the assay may be used as a valuable diagnostic tool for the rapid
and specific detection ofM. haemolytica in clinical samples.
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Date 2021-07-15T09:52:11Z
2021-07-15T09:52:11Z
2019-12-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/48230
 
Language English
 
Relation Not Available;
 
Publisher Not Available