Record Details

Detection of Corynebacterium pseudotuberculosis by SYBR Green Real Time PCR

KRISHI: Publication and Data Inventory Repository

View Archive Info
 
 
Field Value
 
Title Detection of Corynebacterium pseudotuberculosis by SYBR Green Real Time PCR
Not Available
 
Creator Jyoti Kumar, GG Sonawane, S Jegaveera Pandian, Rajiv Kumar
 
Subject Real time PCR, Corynebacterium pseudotuberculosis, sensitivity, specificity, SYBR green
 
Description Not Available
Corynebacterium pseudotuberculosis, a Gram positive bacterium is the causative agent of
caseous lymphadenitis (CLA), a chronic debilitating disease of sheep, goats and other
warm blooded animals. Definite diagnosis of CLA primarily relies on microbiological
examination, followed by biochemical identification of isolates. In an effort to facilitate C.
pseudotuberculosis detection, the present study was undertaken with the aim of evaluating
a SYBR Green real time PCR assay targeting ABC transporter gene for the detection of C.
pseudotuberculosis in DNA isolated from culture and pus samples from sheep and goats.
The analytical sensitivity and specificity of the PCR primers were evaluated. The test
demonstrated tenfold more sensitivity than conventional PCR and detected down to 100
femtogram of genomic DNA of pure C. pseudotuberculosis. The real-time PCR was found
to be specific for ABC transporter gene of C. pseudotuberculosis, as no cross reactivity
was detected with a variety of known bacterial isolates. The real time PCR was employed
for direct detection of C. pseudotuberculosis in pus samples collected from clinically
suspected cases of caseous lymphadenitis. It is concluded that, this real time assay may be
used as a valuable diagnostic tool for the rapid and specific detection of C.
pseudotuberculosis in clinical samples, large scale epidemiological studies and
clarification of pathogenesis.
Not Available
 
Date 2021-07-15T09:52:33Z
2021-07-15T09:52:33Z
2019-02-10
 
Type Article
 
Identifier Not Available
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/48231
 
Language English
 
Relation Not Available;
 
Publisher Not Available