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Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos

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Title Simple multiplex PCR for rapid diagnosis of sex of ducks and duck embryos
Not available
 
Creator Santosh Haunshi,
Saxena, S. C.
Pattanayak, A.
Bandyopadhaya, S.
Tyagi, A.K.
Bujarbaruah, K. M.
 
Subject Multiplex PCR, duck, embryo, sex diagnosis
 
Description Not Available
A simple and rapid multiplex polymerase chain reaction (PCR) was developed for quick diagnosis of sex
of duck and duck embryos. W chromosome specific DNA sequence was selected and primers were designed
to amplify 335 bp fragment from female sex while 16s ribosomal sequence was selected to design primers
to amplify 468 bp PCR products both in male and female sex as an internal control. Nucleotide sequences
of W chromosome specific DNA fragments of Khaki Campbell and Indian Runner breeds of duck were
found to be identical both in size and sequences, The study concluded that the protocol was successful in
precisely identifying the gender of ducklings belonging to both Indian Runner and Khaki Campbell breeds
of ducks and duck embryos
SERC
 
Date 2021-07-17T09:02:38Z
2021-07-17T09:02:38Z
2008-06-01
 
Type Research Paper
 
Identifier Haunshi, S., S. C. Saxena, A. Pattanayak, S. Bandyopadhaya, A. K. Tyagi and K. M. Bujarbaruah. 2008. Simple Multiplex PCR for Rapid Diagnosis of Sex of Ducks and Duck Embryos, Journal of Applied Animal Research, 33:2, 117-120, DOI: 10.1080/09712119.2008.9706911
Not Available
http://krishi.icar.gov.in/jspui/handle/123456789/48568
 
Language English
 
Relation Not Available;
 
Publisher Taylor and Francis