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Effect of in vitro zinc supplementation on HSPs expression and Interleukin 10 production in heat treated peripheral blood mononuclear cells of transition Sahiwal and Karan Fries cows

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Title Effect of in vitro zinc supplementation on HSPs expression and Interleukin 10 production in heat treated peripheral blood mononuclear cells of transition Sahiwal and Karan Fries cows
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Creator Aasif Ahmad Sheikh n , Anjali Aggarwal, Ovais Aarif
 
Subject Sahiwal Karan Fries Transition PBMC Zinc HSP Interleukin
 
Description Not Available
stock adversely vis-à-vis production and reproduction. This has prompted more focus in addressing the
unfavorable effects of thermal stress in livestock system. Presuming that the trace element zinc is in dispensible for cellular antioxidant system and immune function, the present study was designed to
investigate the effect of zinc treatment on heat stress alleviation and immune modulation in peripheral
blood mononuclear cells (PBMC) of indigenous and crossbred transition cows. Twelve cows, six each of
Sahiwal and Karan Fries (KF) in their second parity with confirmed pregnancy were selected for the
experiment. The blood samples were collected at 21, 0 and þ21 days in relation to expected date of
calving. The experiment was carried out in vitro after isolating PBMC from whole blood. The 48 h cultured
PBMC were subjected to assorted levels of exposures viz. 37 °C, 42 °C to impose heat stress and
42 °Cþzinc to alleviate heat stress and modulate immunity. The PBMC viability was 86%, 69% and 78%,
respectively. The mRNA expression of heat shock proteins (HSP 40, 70 and 90α) and Interleukin-10 (IL-
10) production varied between the two breeds vis-à-vis days and levels of exposure. The mRNA ex pression of HSP40 and HSP70 was significantly (Po0.05) higher in Karan Fries than the Sahiwal cows.
Both the breeds showed maximum expression of HSP on the day of parturition, more so in KF than
Sahiwal. There was a significant (Po0.05) difference in the HSP mRNA expression at different levels of
exposure. Zinc treatment to heat stressed PBMC caused a significant (Po0.05) down regulation of HSP.
For immune status, anti-inflammatory cytokine, IL-10 in the culture supernatant was accessed. The IL-10
was significantly (Po0.05) higher in Karan Fries (168.18714.09 pg/ml) than the Sahiwal cows
(147.24711.82 pg/ml). The IL-10 concentration was highest on the day of calving. Zinc treatment reduced
the IL-10 concentration. From the study, it could be concluded that the zinc supplementation in heat
stressed PBMC can ameliorate thermal stress and modulate immune response which can act as a model
for reducing heat stress during the periparturient period in tropical livestock.
& 2016 P
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Date 2021-07-31T10:14:29Z
2021-07-31T10:14:29Z
2016-01-01
 
Type Article
 
Identifier Not Available
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http://krishi.icar.gov.in/jspui/handle/123456789/51714
 
Language English
 
Relation Not Available;
 
Publisher Not Available